The role of tumor necrosis factor-α in spleen infection caused by Leishmania
10.19405/j.cnki.issn1000-1492.2025.12.016
- VernacularTitle:肿瘤坏死因子⁃α 在利什曼原虫脾脏感染中的作用
- Author:
Erna Zhen
1
;
Yuling Wu
1
;
Shanshan Hu
2
Author Information
1. Institute of Clinical Pharmcology, School of Pharmaceutical Science ,Anhui Medical University, Key Laboratory of Anti⁃Inflammatory and Immune Medicine , Hefei 230032
2. Institute of Clinical Pharmcology, School of Pharmaceutical Science ,Anhui Medical University, Key Laboratory of Anti⁃Inflammatory and Immune Medicine , Hefei 230032 ; Dept of Clinical Laboratory, The First Afiliated Hospital of University of Science and Technology of China , Hefei 230036
- Publication Type:Journal Article
- Keywords:
Leishmania;
tumor necrosis factor-α;
spleen;
macrophages;
flow cytometry analysis;
immune response
- From:
Acta Universitatis Medicinalis Anhui
2025;60(12):2325-2332
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the role of tumour necrosis factor-α(TNF-α) in splenic infection of mice by Leishmania major.
Methods:To establish an infection model, promastigotes of Leishmania were injected intradermally into the right hind foot of mice. The thickness of the footpad and body weight were measured to monitor the infection. Histological changes in the spleen after infection were observed by HE staining. Changes in lymphocytes and monocytes in the spleen were detected by flow cytometry. The expression level of arginase-1(Arg-1) and inducible nitric oxide synthase(iNOS) in the spleen was determined by indirect immunofluorescence. The effect of TNF-α on macrophage infection with Leishmania was evaluated in vitro.
Results:Compared to B6.WT mice, the spleens of B6.TNF-/- mice showed significant enlargement 42 days post-infection, with structural disruption. Various cells infiltrated and were dispersed throughout the entire spleen. Flow cytometry results indicated that after infection with Leishmania, there was no significant change in the proportions of T cells and B cells in the spleens of the mice, while CD11b monocytic cells significantly increased. Immunofluorescence results revealed that the M2 macrophage/monocyte marker Arg-1 was highly expressed in the spleens of B6.TNF-/- mice(P < 0. 05) . The expression of iNOS in the spleens of B6. WT mice was relatively strong (P < 0. 05) . In vitro studies found that the absence or inhibition of TNF⁃α significantly increased the infection of Leishmania by peritoneal macrophages ( P <0. 01), while the addition of TNF-a markedly inhibited this infection (P <0. 01).
Conclusion:The splenic infec-tion in B6. TNF mice following subcutaneous inoculation of Leishmania in the hind footpad may be associatedwith the absence of TNF-a, which leads to M2-type differentiation of macrophages and reduced nitric oxide producton.
- Full text:2026030323193456931肿瘤坏死因子-α在利什曼原虫脾脏感染中的作用_甄二娜.pdf
