The impact of the transcription factor ZNF174 on tumor stemness in non-small cell lung cancer
10.19405/j.cnki.issn1000-1492.2025.12.014
- VernacularTitle:转录因子 ZNF174 对非小细胞肺癌肿瘤干性的影响
- Author:
Xueyi Feng
1
;
Qian Dai
1
Author Information
1. School of Life Sciences , Anhui Medical University, Hefei 230032
- Publication Type:Journal Article
- Keywords:
non-small cell lung cancer;
tumor stemness;
transcription factor;
ZNF174;
lung cancer;
tumor
- From:
Acta Universitatis Medicinalis Anhui
2025;60(12):2308-2315
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of transcription factor ZNF174 on the stemness of non-small cell lung cancer(NSCLC).
Methods:StemChecker online analysis was employed to assess the impact of ZNF174 on tumor stemness markers. The ZNF174 knockdown plasmid was constructed via restriction enzyme digestion and enzyme-linked assembly, while the ZNF174 overexpression plasmid was generated through homologous recombination. Lentiviral infection established stable cell lines with ZNF174 knockdown or overexpression. Tumor stem cell spheroid formation assays were conducted to evaluate stemness maintenance capacity. RNA-seq and quantitative real-time polymerase chain reaction(qPCR) were utilized to detect effects on key stemness gene expression.
Results:The transcription factor ZNF174 promoted cellular stemness in non-small cell lung cancer(NSCLC). It promoted the expression of key stemness genes: B-cell-specific moloney murine leukemia virus insertion site 1(BMI1), acetaldehyde dehydrogenase 2(ALDH2), SRY-box transcription factor 2(SOX2), recombinant high mobility group AT hook protein 2(HMGA2), and snail family transcriptional repressor 1(Snail1) and enhanced tumor spheroid formation in NSCLC stem cells.
Conclusion:ZNF174 promotes the expression of tumor stemness genes in NSCLC and may become a novel therapeutic and prognostic target for NSCLC.
- Full text:2026030323084546814转录因子ZNF174对非小细胞肺癌肿瘤干性的影响_冯学溢.pdf
