Investigating the mechanism of Xanthatin in inhibiting proliferation of laryngeal squamous cell carcinoma cells based on network pharmacology , transcriptomics , and experimental validation
10.19405/j.cnki.issn1000-1492.2025.12.012
- VernacularTitle:基于网络药理学、转录组学和实验验证探讨 苍耳亭抑制喉鳞状细胞癌细胞增殖的作用机制
- Author:
Zichuang Ma
1
;
Dan Su
2
;
Chun Wang
3
;
Na Wu
4
;
Haikun Wang
5
;
Aizong Shen
6
Author Information
1. School of Pharmaceutical Sciences , Anhui Medical University, Hefei 230032
2. Health Management Center, The First Afiliated Hospital of University of Science and Technology of China , Hefei 230001
3. Institute of Clinical Pharmacology, School of Pharmaceutical Sciences , Anhui Medical University, Hefei 230032
4. Dept of Pharmacy, Bozhou Hospital of Anhui Medical University, Bozhou 236800
5. School of Pharmaceutical Sciences , Anhui Medical University, Hefei 230032 ;Dept of Pharmacy, Bozhou Hospital of Anhui Medical University, Bozhou 236800
6. Dept of Pharmacy, the First Afiliated Hospital of University of Science and Technology of China , Hefei 230001
- Publication Type:Journal Article
- Keywords:
network pharmacology;
Xanthatin;
laryngeal squamous cell carcinoma;
molecular docking;
cell experiments;
PI3K-Akt signaling pathway;
transcriptomics;
experimental validation
- From:
Acta Universitatis Medicinalis Anhui
2025;60(12):2289-2298
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the potential mechanisms of Xanthatin in inhibiting the proliferation of laryngeal squamous cell carcinoma(LSCC) cells by integrating network pharmacology and in vitro experiments.
Methods:The targets of Xanthatin were identified using databases such as PharmMapper, while disease-related targets for LSCC were obtained from databases such as DisGeNET. The overlapping targets between Xanthatin and LSCC were determined by intersecting these datasets. A protein-protein interaction(PPI) network was constructed based on the overlapping targets, and key targets were identified. Gene ontology(GO) and Kyoto encyclopedia of genes and genomes(KEGG) enrichment analyses of the overlapping targets were performed using R software. A "Xanthatin-target-pathway" network was visualized using Cytoscape 3.8.0 software. The preliminary validation of the aforementioned results was performed using molecular docking and transcriptomics. The effects of Xanthatin on the proliferation of TU177 cells were assessed using CCK-8 and colony formation assays. Additionally, Western blot analysis was employed to measure the expression levels of PI3K, p-PI3K, Akt, and p-Akt proteins.
Results:A total of 159 overlapping targets between Xanthatin and LSCC were identified, and seven key targets, including AKT1, were screened. GO enrichment analysis yielded 2 455 entries, and KEGG enrichment analysis identified 172 pathways, such as the PI3K-Akt signaling pathway. Xanthatin exhibited favorable binding activity with the core target proteins of LSCC in molecular docking experiments. The transcriptomics results showed high consistency with the predictions from network pharmacology. CCK-8 and colony formation assays demonstrated that Xanthatin at concentrations of 1, 2, and 4 μmol/L significantly inhibited the proliferation of TU177 cells in a dose-dependent manner. The expression levels of p-PI3K and p-Akt proteins decreased with increasing concentrations of Xanthatin.
Conclusion:Xanthatin may exert an inhibitory effect on the proliferation of LSCC cells by modulating the PI3K-Akt signaling pathway.
- Full text:2026030323000415191基于网络药理学、转录组学和实验验证探讨苍耳亭抑制喉鳞状细胞癌细胞增殖的作用机制_马自创.pdf
