Bioinformatics analysis of differentially expressed genes in DCX-overexpression glioma cells
10.19405/j.cnki.issn1000-1492.2025.12.007
- VernacularTitle:DCX 过表达胶质瘤细胞中差异基因的生物信息学分析
- Author:
Lingxin Zhou
1
;
Iqra Nadeem
2
;
Yuxia Qin
1
;
Ye Xiong
1
Author Information
1. School of Life Sciences ,Xuzhou Medical University,Xuzhou 221004
2. School of Basic Medical Sciences ,Xuzhou Medical University,Xuzhou 221004
- Publication Type:Journal Article
- Keywords:
glioma;
doublecortin;
differentially expressed genes;
bioinformatics;
enrichment analysis;
RNA sequencing
- From:
Acta Universitatis Medicinalis Anhui
2025;60(12):2247-2254
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role mechanism of doublecortin(DCX) in glioma and screen DCX overexpression-related differentially expressed genes, so as to provide new targets for glioma targeted therapy.
Methods:Using rat glioma C6 cells as the target cells, LV-DCX-EGFP, LV-Cas9-Puro, and LV-Ctrl-EGFP lentiviruses were constructed. DCX overexpressing and control C6 cell lines were established through Cas9 dual-vector system transfection and screening. Transcriptome sequencing was used to obtain the differentially expressed gene profiles. iDEP and DESeq2 were applied to screen differentially expressed genes with the threshold of |Log_2FC|>2 and P0.7, indicating good diagnostic value. RT-qPCR validation showed that the mRNA expression levels of some upregulated genes(POU2F3, LPAR6, SREBF1) significantly increased ( P < 0. 001) , while the mRNA expression levels of some downregulated genes (UBB , ACTB , UBE2I) also significantly increased (P < 0. 001) , which might be relat⁃ed to transcriptional and post⁃transcriptional regulation.
Conclusion:Differentially expressed genes and their regu⁃latory networks related to DCX overexpression in glioma are successfully screened , providing a theoretical basis for revealing the role mechanism of DCX in glioma development and laying a foundation for the development of potential therapeutic strategies.
- Full text:202603032227451352DCX过表达胶质瘤细胞中差异基因的生物信息学分析_周玲欣.pdf
