Explore the causal association between antibody immune response and ulcerative colitis based on Mendelian randomization
10.19405/j.cnki.issn1000-1492.2025.06.018
- Author:
Yixuan Zeng
1
;
Niren Li
1
;
Bingying Deng
1
;
Pai Xie
1
;
Rihong Ou
1
;
Lei Chen
2
;
Yi Liu
1
Author Information
1. Guangdong Provincial Key Laboratory of Chinese Medicine Pharmaceutics , Guangdong Basic Research Center of Excellence for Integrated Traditional and Western Medicine for Qingzhi Diseases , School of Traditional Chinese Medicine , Southern Medical University , Guangzhou 510515
2. School of Traditional Chinese Medicine , Guangdong Pharmaceutical University , Guangzhou 510006
- Publication Type:Journal Article
- Keywords:
antibody level;
ulcerative colitis;
Mendelian randomization;
causal association;
EB virus;
risk prediction
- From:
Acta Universitatis Medicinalis Anhui
2025;60(6):1098-1104
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the causal relationship between 46 phenotypes ( including 15 seropositive case- control phenotypes and 31 quantitative antibody-measurement phenotypes) and ulcerative colitis( UC) using two- sample bidirectional Mendelian randomization( TSMR) .
Methods:Single nucleotide polymorphisms ( SNPs) sig- nificantly associated with the relative abundance of the 46 antibody sera were extracted as instrumental variables ac- cording to preset thresholds . Summary statistics for UC were obtained from the OPEN GWAS database ( n = 47 745) . MR-Egger regression , weighted median method ( WME) , inverse variance weighting ( IVW) , the simple mode method (SM) , and weighted multitude method (WM) were used to estimate the causal relationship between antibody levels and UC , primarily using the IVW method . The results were assessed according to the effect indica- tor dominance ratios (OR) and 95% confidence intervals (CI) . Sensitivity analysis , heterogeneity test , gene plei- otropy test , and outlier test (MR-PRESSO) were combined to verify the stability and reliability of the results , and the causal association study was performed again using reverse Mendelian randomization(MR) .
Results :IVW re- sults showed that Epstein-Barr( EB) virus EA-D antibody levels ( OR = 0. 806 , 95% CI = 0. 693 - 0. 939 , P < 0. 01) , Epstein-Barr virus EBNA-1 antibody levels ( OR = 1 . 870% , 95% CI = 1 . 480 - 2. 360 , P < 0. 000 1) , Anti-polyomavirus 2 IgG seropositivity (OR = 0. 570 , 95% CI = 0. 435 - 0. 746 , P < 0. 000 1) were associated with UC . The inverse MR analysis revealed a causal effect on anti-polyomavirus 2 IgG seropositivity , and none of the a- bove revealed genetic pleiotropy or significant heterogeneity of IVs .
Conclusion:EB virus EBNA-1 antibody levels are positively associated with the risk of UC , while EB virus EA-D antibody levels and anti-polyomavirus 2 IgG se- ropositivity are negatively associated with the risk of UC , indicating that they are protective factors for UC .
- Full text:2026030316211649265基于孟德尔随机化探讨抗体免疫反应与溃疡性结肠炎的因果关联_曾译萱.pdf
