Endoplasmic reticulum stress involved in high-fat induced metabolic dysfunction-associated steatotic liver disease
10.19405/j.cnki.issn1000-1492.2025.06.016
- Author:
Weikang Hu
1
;
Wenjing Zhou
1
;
Jun Zhang
1
;
Zhen Yang
1
;
Hongmei Bai
1
;
Zihan Wang
1
;
Yinliang Qi
2
;
Shengquan Zhang
1
;
Sumei Zhang
1
Author Information
1. Dept of Biochemistry and Molecular Biology , School of Basic Medicine , Anhui Medical University , Hefei 230032
2. Afiliated Hefei Hospital of Anhui Medical University Hefei (The Second People ′sHospital of Hefei) , Hefei 230012
- Publication Type:Journal Article
- Keywords:
endoplasmic reticulum stress;
high fat;
MASLD;
Drosophila;
palmitic acid;
seatosis
- From:
Acta Universitatis Medicinalis Anhui
2025;60(6):1086-1090
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the role of endoplasmic reticulum stress in the occurrence and development of fatty liver induced by high fat.
Methods :In the high-fat Drosophila model, the high-fat group was fed with high-fat medium, while the control group was fed with normal medium; in the mouse fatty liver model, the high-fat group was fed with high-fat diet, and the control group was fed with normal diet; in the HepG2 cell steatosis model, the high-fat group was induced by palmitic acid(PA), and the control group was cultured with DMEM. The fat body size of the third instar larvae of Drosophila melanogaster was photographed. Steatosis in mice liver and HepG2 cells was observed by H&E and Oil Red staining. The expression levels of ATF6, Bip and CHOP in the third instar larvae, liver tissues of mice and HepG2 cells were analyzed by quantitative real-time polymerase chain reaction(qPCR) and Western blot.
Results :In Drosophila model, fat body and fat storage were obviously increased in high fat fed flies when compared with control group. The formation of liver fat droplets and cells vacuolation were confirmed by H&E and Oil Red staining in mice livers fed with high fat and HepG2 cells with palmitic acid treatment. The expression levels of ATF6, Bip and CHOP were significantly increased in third instar larvae and mice livers fed with high fat and palmitic acid treated HepG2 cells with palmitic acid treatment.
Conclusion :High fat may induce the occurrence and development of hepatic steatosis by activating endoplasmic reticulum stress.
- Full text:2026030316003106185内质网应激在高脂诱导的代谢障碍相关脂肪变性肝病中的作用_胡韦康.pdf
