The role of BRD4 in HPV16 virus replication in cervical tissue and cells
10.19405/j.cnki.issn1000-1492.2025.06.015
- Author:
Le Wang
1
;
Weixin Li
1
;
Yangliu Dong
1
;
Xian Zhao
1
;
Xinli Zhu
1
;
Xuechen Zhang
1
;
Xiangyi Zhe
1
;
Zemin Pan
1
Author Information
1. Key Laboratory of Xinjiang Endemic and Ethnic Diseases , School of Medicine , Shihezi University , Shihezi 832002
- Publication Type:Journal Article
- Keywords:
cervical cancer;
bromodomain protein 4;
human papillomavirus16;
viral load;
MZ1
- From:
Acta Universitatis Medicinalis Anhui
2025;60(6):1080-1085
- CountryChina
- Language:Chinese
-
Abstract:
Abstract:To explore the relationship between the replication-associated bromodomain protein 4 ( BRD4)and human papillomavirus (HPV) 16(HPV16) viral load in cervical squamous cell carcinoma and CIN Ⅰ tissues , confirm the effects of BRD4 degradation agent MZ1 on viral load .
Methods : Thirty HPV16-positive cervi- cal cancer specimens and 30 non-cervical cancer specimens were collected , and the viral load of the samples was detected by real-time fluorescence quantitative PCR , and the expression of BRD4 was analyzed by immunohisto- chemistry and Western blot.
Results :The viral load was higher in the samples of cervical cancer group than in the samples of non-cancer group , the difference is statistically significant ( P < 0. 01) . Immunohistochemistry results showed that the expression of BRD4 were significantly higher in cervical cancer specimens than in noncancerous specimens , the difference is statistically significant (P < 0. 05) . BRD4 expression was significantly and positively correlated with high viral loads , the difference is statistically significant (P < 0. 001) . the BRD4 degradation agent MZ1 significantly reduced the viral load , the difference is statistically significant (P < 0. 01) .
Conclusion : BRD4 may be involved in the replication of HPV16 virus , and BRD4 degradation agent MZ1 can inhibit the replication of HPV16 virus .
- Full text:2026030315565468284宫颈组织和细胞中BRD4在HPV16病毒复制中的作用_王乐.pdf
