The expression and interaction of ATF3 and Smad4 in pterygium
10.19405/j.cnki.issn1000-1492.2025.06.013
- Author:
Yun Hua
1
;
Huanhuan Han
2
;
Dongmei Chen
3
;
Jialin Liu
3
;
Xinrong Zhao
1
Author Information
1. Dept of Ophthalmology , the First Afiliated Hospital of Shihezi University , Shihezi 832003
2. Dept of Ophthalmology , the First Afiliated Hospital of Shihezi University , Shihezi 832003 ; Shihezi University School of Medicine , Shihezi 832003
3. Shihezi University School of Medicine , Shihezi 832003
- Publication Type:Journal Article
- Keywords:
pterygium;
ATF3;
Smad4;
interaction;
preliminary investigation
- From:
Acta Universitatis Medicinalis Anhui
2025;60(6):1068-1073
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the expression and interaction of activating transcription factor 3(ATF3) and smad family member 4(Smad4) in pterygium.
Methods:In this study, two sets of data GSE51995 and GSE2513 in NCBI database were analyzed by bioinformatics methods to screen out the key difference-expressed gene ATF3 in pterygium when normal conjunctiva was used as the control. The differential genes identified by bioinformatics analysis were further detected by reverse transcription quantitative polymerase chain reaction(RT-qPCR). Finally, the literatures were searched and The Gene Transcription Regulation Database(GTRD) was used to predict whether there was a target gene Smad4 that might bind to the target gene. The expression of Smad4 in normal conjunctival and pterygium tissues was verified by RT-qPCR, and the interaction between the target protein ATF3 and Smad4 in the pterygium was investigated through Co-Immunoprecipitation(Co-IP).
Results :The results of bioinformatics a- nalysis showed that compared with normal conjunctival tissue , there was significantly different expression of ATF3 gene in pterygium tissue (P < 0. 05) . RT-qPCR confirmed that ATF3 was less expressed in pterygium tissue than normal conjunctival tissue (P < 0. 001) . Genomic data from the GTRD database revealed that Smad4 contains two ATF3 binding motifs , suggesting functional interaction potential . Compared with normal conjunctiva , RT-qPCR re- vealed Smad4 downregulation in pterygium tissues (P < 0. 01) ; Co-IP demonstrated enhanced interaction between Smad4 and ATF3 in pterygium tissues following immunoprecipitation with anti-ATF3 antibodies (P < 0. 05) .
Conclusion :ATF3 interacts with Smad4 in pterygium , and the low expression of both in pterygium tissues and their in- teraction may be associated with the pathogenesis of pterygium .
- Full text:2026030315414276428ATF3与Smad4在翼状胬肉中的表达及相互作用_华耘.pdf
