Preliminary exploration of the mechanism of action of Brassica rapa L. in treating pulmonary fibrosis based on network pharmacology and animal experiments
10.19405/j.cnki.issn1000-1492.2025.12.005
- VernacularTitle:基于网络药理学和动物实验初探 芜菁治疗肺纤维化的作用机制
- Author:
Mingyu Sun
1
;
Guihua Liu
2
;
Junting Guo
2
;
Aibin Cheng
3
;
Jing Xin
1
;
Qingfang Miao
4
;
Ruijuan Gao
4
;
Xiuli Men
5
Author Information
1. Key Laboratory of Chronic Diseases , School of Basic Medical Sciences , North China University of Science and Technology, Tangshan 063210;NHC Key Laboratory of Biotechnology for Microbial Drugs , Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College , Beijing 100050
2. Food Testing Research Laboratory,Xinjiang Uygur Autonomous Region Institute of Materia Medica , Urumqi 830000
3. Intensive Care Unit ,North China University of Science and Technology Afiliated Hospital , Tangshan 063210
4. NHC Key Laboratory of Biotechnology for Microbial Drugs , Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College , Beijing 100050
5. Key Laboratory of Chronic Diseases , School of Basic Medical Sciences , North China University of Science and Technology, Tangshan 063210
- Publication Type:Journal Article
- Keywords:
Brassica rapa L.;
polysaccharide ofBrassica rapa L.;
pulmonary fibrosis;
network pharmacology;
tumor necrosis factor-α;
PI3K-Akt signaling pathway;
TNF-α signaling pathway
- From:
Acta Universitatis Medicinalis Anhui
2025;60(12):2227-2234
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the active components, key targets, and mechanism of action of turnip in alleviating pulmonary fibrosis(PF) based on network pharmacology and animal experiments.
Methods:The active components and targets of Brassica rapa L. were screened using the traditional Chinese medicine systems pharmacology database and analysis platform database, and PF-related targets were obtained from disease databases such as online mendelian inheritance of man(OMIM) and DrugBank. The intersection targets were used to construct a protein-protein interaction(PPI) network to identify core targets, followed by gene oncology(GO)/Kyoto encyclopedia of genes and genomes(KEGG) pathway enrichment analysis. In the animal experiments, a bleomycin-induced PF mouse model was established. Pathological changes in lung tissue were evaluated using HE and Masson staining. qRT-PCR was used to detect the mRNA expression of tumor necrosis factor-α(TNF-α), phosphatidylinositol 3-kinase(PI3K), and akstrain transforming 1(AKT1), and immunofluorescence staining was used to measure the protein expression of TNF-α, PI3K, and AKT1.
Results:The 68 active components identified in Brassica rapa L. may regulate PI3K-Akt signaling pathway by acting on 89 potential targets such as TNF-α and AKT1. The results of animal experiments showed that polysaccharide of Brassica rapa L.(BRPs) could significantly reduce the degree of bleomycin induced pulmonary fibrosis in mice; HE and Masson staining of lung tissue showed that compared with the model group, the damage of alveolar structure, the infiltration of inflammatory cells and the deposition of collagen fibers in the BRPs treatment group were significantly reduced. Further mechanism studies showed that BRPs could significantly down-regulate the mRNA and protein expression levels of TNF-α, PI3K and AKT1 in lung tissue of pulmonary fibrosis mice.
Conclusion:Brassica rapa L. can synergistically alleviate pulmonary fibrosis through “multi-component, multi-target and multi-channel” approach; BRPs is one of the main active components, and plays an anti-fibrosis role by inhibiting TNF-α/PI3K Akt signaling pathway.
- Full text:2026030300075111045基于网络药理学和动物实验初探芜菁治疗肺纤维化的作用机制_孙铭雨.pdf
