Effect of LncRNA RMRP on ferroptosis induced by oxygen glucose deprivation/reperfusion in mouse HL-1 cardiomyocytes by regulating miR-766-5p
10.19405/j.cnki.issn1000-1492.2025.12.003
- VernacularTitle:LncRNA RMRP 通过调控 miR⁃766⁃5p 对 氧糖剥夺／再灌注诱导的小鼠 HL⁃1 心肌细胞铁死亡的影响
- Author:
Lei He
1
;
Xinglan Sun
1
;
Yingxing Wu
1
;
Yuan Xu
2
;
Xiang Peng
3
;
Chenkai Hu
1
Author Information
1. Dept of Cardiology,The Second Afiliated Hospital , Jiangxi Medical College , Nanchang University, Nanchang 330006
2. Medical Big Data Research Center,The Second Afiliated Hospital , Jiangxi Medical College , Nanchang University, Nanchang 330006
3. Information Department ,The Second Afiliated Hospital , Jiangxi Medical College , Nanchang University, Nanchang 330006
- Publication Type:Journal Article
- Keywords:
oxygen-glucose deprivation/reperfusion;
myocardium;
HL-1 cells;
ferroptosis;
long non-coding RNA RMRP;
miR-766-5p
- From:
Acta Universitatis Medicinalis Anhui
2025;60(12):2207-2214
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect and mechanism of long non-coding RNA RMRP(LncRNA RMRP) on oxygen-glucose deprivation/reperfusion(OGD/R)-induced ferroptosis in mouse HL-1 cardiomyocytes by regulating miR-766-5p.
Methods:HL-1 cells were cultured in vitro, and OGD/R models were established. The expression levels of LncRNA RMRP in HL-1 cells at various reperfusion time points were subsequently quantified using qRT-PCR. The LncRNA RMRP small RNA interference fragment(si-RMRP) and its corresponding negative control(si-NC), as well as the miR-766-5p inhibitor and its respective negative control(inhibitor-NC), were transfected into HL-1 cells. Subsequently, the cells were subjected to OGD/R treatment. CCK-8 assay was employed to evaluate cell viability. Assay kits were employed to measure the levels of lactate dehydrogenase(LDH) in the cell supernatant, as well as the intracellular levels of malondialdehyde(MDA), superoxide dismutase(SOD), glutathione(GSH), and ferrous ion(Fe2+). qRT-PCR analysis was conducted to assess the expression levels of LncRNA RMRP and miR-766-5p. Western blot analysis was conducted to assess the expression levels of proteins associated with ferroptosis including GPX4, SLC7A11, and FTH1. Dual-luciferase reporter assays were performed to investigate the sponge adsorption relationship between LncRNA RMRP and miR-766-5p.
Results:As reperfusion time extended, the expression level of LncRNA RMRP in cells progressively increased(P2+ levels within the cells, and decreased the activities of SOD and GSH in cells(P2+ levels within the cells, and promoting SOD and GSH activities in cells(PP < 0. 01) . Furthermore , silencing LncRNA RMRP upregulated the protein expression levels of GPX4 , SLC7A11 , and FTH1(P < 0. 01) . The dual-luciferase reporter assay confirmed that LncRNA RMRP could regulate the expression of miR-766- 5p through a sponge adsorption mechanism. Partial inhibition of miR-766-5p inhibitor expression could mitigate the improvement effect caused by LncRNA RMRP silencing on OGD/R-induced ferroptosis in HL-1 cells.
Conclusion:Silencing LncRNA RMRP inhibits OGD/R-induced ferroptosis in HL-1 cells , potentially through the sponge-mediated regulation of miR-766-5p expression.
- Full text:2026030223393038232LncRNA_RMRP通过调控miR-766-5p对...糖剥夺_再灌注诱导的小鼠HL-1心肌细胞铁死亡的影响_何蕾.pdf
