Evaluation of acute kidney injury model in rats induced by different doses of vancomycin
10.19405/j.cnki.issn1000-1492.2025.06.008
- VernacularTitle:不同剂量万古霉素诱导大鼠急性肾损伤模型的评价
- Author:
Fei MA
1
;
Qinxiang DENG
;
Rui SHI
;
Chun WANG
;
Bingfa XU
Author Information
1. 安徽医科大学药学科学学院,合肥 230032;安徽医科大学第三附属医院药学部,合肥 230032
- Publication Type:Journal Article
- Keywords:
acute kidney injury;
animal model;
nephrotoxicity;
vancomycin;
dose;
rat
- From:
Acta Universitatis Medicinalis Anhui
2025;60(6):1030-1035
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the method and evaluation of using different doses of vancomycin(VCM)to construct an acute kidney injury(AKI)model in SD rats.Methods The 6-week-old male SD rats were randomly divided into 4 groups:normal control(NC)group,VCM(200,400,600)mg/kg group.The drug was given by intraperitoneal injection once a day for 7 days.The levels of blood urea nitrogen(BUN)and creatinine(Cre)were detected.The kidney was weighed and the kidney body ratio was calculated.Renal tissue was stained with HE and PAS,and the pathological injury of kidney was observed under light microscope.Results With the increase of VCM dose,serum BUN and Cre levels in each dose group showed an upward trend(F=3.85,P＜0.05;F=9.02,P＜0.01).Compared with the VCM(200,400)mg/kg groups,the Cre level in the VCM 600 mg/kg group was the highest,with statistically significant differences(P＜0.01,P＜0.05).As the VCM dose in-creased,body weight of rats in each dose group decreased(F=54.81,P＜0.01),while total kidney weight and kidney body ratio increased(F=11.13,F=14.43,both P＜0.01).Significant statistically differences(P＜0.01,P＜0.05)were observed in body weight,total kidney weight and kidney body ratio between the VCM 600 mg/kg group and the VCM(200,400)mg/kg groups.HE staining and PAS staining showed that renal tubules in each dose group were damaged to varying degrees.With the increase of VCM dose,the pathological score of kidney injury increased(F=105.80,F=28.89,both P＜0.01),and the pathological damage of renal tubular epithelial cells such as shedding,swelling and cast formation was the most serious in VCM 600 mg/kg group.Conclusion The AKI model in rats can be stably established by intraperitoneal injection of VCM at 600 mg/kg once a day for 7 days.
