Evaluation of acute kidney injury model in rats induced by different doses of vancomycin
- Author:
Fei Ma
1
;
Qinxiang Deng
1
;
Rui Shi
1
;
Chun Wang
2
;
Bingfa Xu
1
Author Information
1. School of Pharmaceutical Sciences , Anhui Medical University , Hefei 230032 ; Dept of Pharmacy , The Third Afiliated Hospital of Anhui Medical University , Hefei 230032
2. School of Pharmaceutical Sciences , Anhui Medical University , Hefei 230032
- Publication Type:Journal Article
- Keywords:
acute kidney injury;
animal model;
nephrotoxicity;
vancomycin;
dose;
rat
- From:
Acta Universitatis Medicinalis Anhui
2025;60(6):1030-1035
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the method and evaluation of using different doses of vancomycin(VCM) to construct an acute kidney injury(AKI) model in SD rats.
Methods :The 6-week-old male SD rats were randomly divided into 4 groups: normal control(NC) group, VCM(200, 400, 600) mg/kg group. The drug was given by intraperitoneal injection once a day for 7 days. The levels of blood urea nitrogen(BUN) and creatinine(Cre) were detected. The kidney was weighed and the kidney body ratio was calculated. Renal tissue was stained with HE and PAS, and the pathological injury of kidney was observed under light microscope.
Results :With the increase of VCM dose, serum BUN and Cre levels in each dose group showed an upward trend(F=3.85,P<0.05;F=9.02,P<0.01). Compared with the VCM(200, 400) mg/kg groups, the Cre level in the VCM 600 mg/kg group was the highest, with statistically significant differences(P<0.01,P<0.05). As the VCM dose increased, body weight of rats in each dose group decreased(F=54.81,P<0.01), while total kidney weight and kidney body ratio increased(F=11.13,F=14.43, bothP<0.01). Significant statistically differences(P<0.01,P<0.05)were observed in body weight, total kidney weight and kidney body ratio between the VCM 600 mg/kg group and the VCM(200, 400) mg/kg groups. HE staining and PAS staining showed that renal tubules in each dose group were damaged to varying degrees. With the increase of VCM dose, the pathological score of kidney injury increased(F=105.80,F=28.89, bothP<0.01), and the pathological damage of renal tubular epithelial cells such as shedding, swelling and cast formation was the most serious in VCM 600 mg/kg group.
Conclusion :The AKI model in rats can be stably established by intraperitoneal injection of VCM at 600 mg/kg once a day for 7 days.
- Full text:2026030216103390679不同剂量万古霉素诱导大鼠急性肾损伤模型的评价_马菲.pdf
