Silencing circ FADS2 induces ferroptosis in colorectal cancer cells through miR - 152 - 3p / SLC7A11 signaling axis
10.19405/j.cnki.issn1000-1492.2025.06.004
- Author:
Liangjun Jiang
1
;
Xianzhou Lu
2
Author Information
1. Dept of Gastroenterology , The Afiliated Nanhua Hospital , Hengyang Medical School , University of South China , Hengyang 421002
2. Dept of Hepatobiliary Surgery , The Afiliated Nanhua Hospital , Hengyang Medical School , University of South China , Hengyang 421002
- Publication Type:Journal Article
- Keywords:
circFADS2;
colorectal cancer;
SW480 cells;
death of iron;
miR-152-3p;
solute carrier family 7 member 11
- From:
Acta Universitatis Medicinalis Anhui
2025;60(6):1000-1008
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effect and mechanism of silencing circFADS2 on ferroptosis in colorectal cancer (CRC) cells .
Methods:The human colon adenocarcinoma cell line SW480 was used as the research ob- ject. circFADS2 siRNA interference plasmid (si-circFADS2) and its negative control si-NC , miR-152-3p inhibitor and its negative control inhibitor-NC , miR-152-3p mimics and its negative control mimics NC , SLC7A11 overex- pression plasmid (oe-SLC7A11) and its negative control vector were transfected into SW480 cells . Methylthiazolyl- diphenyl-tetrazolium bromide (MTT) method was used to detect cell proliferation activity; The contents of ferrous ion (Fe2 + ) , malondialdehyde (MDA) and the activities of superoxide dismutase (SOD) and glutathione (GSH) in cells were detected by chemical method . Dichlorodihydrofluorescein diacetate ( DCFH-DA) probe was used to detect the level of reactive oxygen species ( ROS) in cells . Quantitative Real-time polymerase chain reaction (qPCR) was used to detect the expression levels of miR-152-3p and SLC7A11 mRNA in cells . Western blot was used to detect the expression levels of ferroptosis-related proteins such as SLC7A11 and GPX4 in cells . Dual lucif- erase reporter gene experiment was used to verify the sponge adsorption relationship between miR-152-3p and circFADS2 , and the targeted regulation relationship between miR-152-3p and SLC7A11 .
Results :Compared with blank group , the proliferation activity of si-circFADS2 group decreased , the levels of Fe2 + , MDA and ROS in- creased , and the levels of SOD and GSH decreased; At the same time , the expression level of miR-152-3p in- creased , and the protein expression levels of SLC7A11 and GPX4 decreased in cells ( all P < 0. 05) . Compared with si-circFADS2 group , the proliferation activity of si-circFADS2 + inhibitor group increased , the levels of Fe2 + , MDA and ROS in cells decreased , and the levels of SOD and GSH increased; At the same time , the expression level of miR-152-3p decreased , and the protein expression levels of SLC7A11 and GPX4 increased (all P < 0. 05) . The results of dual luciferase reporter gene experiment showed that SLC7A11 was a downstream target gene of miR- 152-3p .
Conclusion :Silencing circFADS2 can induce ferroptosis in CRC cells possibly by targeting the miR-152 - 3p/SLC7A11 signaling axis .
- Full text:2026030208434142751沉默circFADS2通过miR-152-3p_SLC7A11信号轴诱导结直肠癌细胞铁死亡_蒋良君.pdf
