Preliminary study on the antibacterial material basis of Coptidis Rhizoma extracts by different methods based on spectrum-effect relationship
10.12206/j.issn.2097-2024.202403054
- VernacularTitle:基于谱效关系的黄连不同方法提取物抑菌作用物质基础初步研究
- Author:
Xiaoxia NI
1
;
Qiaoxiu CHEN
1
;
Yuru YANG
1
;
Yixiang CAO
1
Author Information
1. Department of Pharmacy, The 909th Hospital / Dongnan Hospital of Xiamen University, Zhangzhou 363000, China.
- Publication Type:Originalarticles
- Keywords:
Coptidis Rhizoma;
spectrum-effect relationship study;
pharmacodynamic substance basis;
antibacterial action;
Staphylococcus aureus
- From:
Journal of Pharmaceutical Practice and Service
2026;44(2):80-84
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the HPLC fingerprints for the different extracts from Coptidis Rhizoma，and investigate the spectrum-effect relationship between HPLC fingerprints and anti-Staphylococcus aureus activity in vitro to analyze the pharmacodynamic material basis. Methods Nine kinds of Coptidis Rhizoma extracts were prepared, and establish the HPLC fingerprints for them. The antibacterial rate of each extract was determined by the broth microdilution method with Staphylococcus aureus as the test bacteria. The grey relational analysis （GRA） method was used to analyze the correlation between the fingerprint data and the in vitro antibacterial test data. Results The HPLC fingerprints of nine kinds of Coptidis Rhizoma extracts were established, nine common characteristic fingerprint peaks were calibrated, and 5 peaks were identified by the reference substance comparison method. GRA analysis experiments showed that the correlation between nine peaks and antibacterial effect was 0.559 1~0.803 3; and the peak 3, peak 8 （palmatine hydrochloride）, peak 9 （berberine hydrochloride） were positively correlated with the inhibition of Staphylococcus aureus,while the peak 9 （berberine hydrochloride） had the strongest effect, and its correlation degree was 0.803 3. Conclusion The effective substance of antibacterial was preliminarily determined through the study of spectrum-effect relationship for Coptidis Rhizoma extracts,which may be the alkaloids, mostly containing hydrochloride, which provided a reference for further research on the pharmacodynamic material basis of Coptidis Rhizoma.
