Research status of protein N-glycosylation detection methods and their applications in ophthalmology research
10.3980/j.issn.1672-5123.2026.3.16
- VernacularTitle:蛋白质N-糖基化检测方法的研究现状及其在眼科研究中的应用
- Author:
Peiyi PENG
1
;
Qingping LIU
1
;
Mingzhi ZHANG
1
Author Information
1. Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou 515041, Guangdong Province, China; Shantou University Medical College, Shantou 515041, Guangdong Province, China
- Publication Type:Journal Article
- Keywords:
N-glycosylation;
glycan analysis;
glycomics;
eye diseases;
mass spectrometry;
liquid chromatography
- From:
International Eye Science
2026;26(3):458-462
- CountryChina
- Language:Chinese
-
Abstract:
N-glycosylation is a crucial posttranslational modification of proteins that can modulate their functions and plays key roles in various biological processes. Current research has revealed that alterations in protein N-glycosylation are closely associated with the pathogenesis and progression of multiple ocular diseases. N-glycosylation detection methods can identify changes in N-glycans on proteins; therefore, understanding these techniques and their application value in ocular diseases is important. This article provides a systematic review of the current state of N-glycosylation detection methods, including techniques such as capillary electrophoresis, liquid chromatography, and mass spectrometry. It also offers a detailed discussion on the specific applications of these technologies in the study of various ocular diseases, including diabetic retinopathy, age-related macular degeneration and vernal keratoconjunctivitis/atopic keratoconjunctivitis.
