Effect of dihydroquercetin on refractive development in mice with form deprivation myopia based on NLRP3 inflammasome pathway
10.3980/j.issn.1672-5123.2026.3.05
- VernacularTitle:基于NLRP3炎症小体途径探讨二氢槲皮素对形觉剥夺性近视小鼠屈光发育的影响
- Author:
Po ZHAO
1
;
Meng MA
1
;
Tengwen YANG
1
;
Lingfei MENG
1
;
Jing LIU
1
;
Xiaoxiao XING
1
;
Huiying HOU
1
Author Information
1. Department of Ophthalmology, Baoding Maternal and Child Health Hospital, Baoding 071000, Hebei Province, China
- Publication Type:Journal Article
- Keywords:
form deprivation myopia;
dihydroquercetin;
NOD-like receptor thermoprotein domain-related protein 3(NLRP3)inflammasome;
pyroptosis;
oxidative stress
- From:
International Eye Science
2026;26(3):391-397
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the effect of dihydroquercetin on visual function in mice with form deprivation myopia based on the NOD-like receptor thermoprotein domain-related protein 3(NLRP3)inflammasome pathway.METHODS: The C57BL/6 mice were randomly divided into control group and form deprivation myopia model group, and the form deprivation myopia model group was constructed by covering the right eye with a translucent eye patch. After successful modeling, the mice in the model group of form deprivation myopia were randomly divided into model group, low-, medium- and high-dose dihydroquercetin groups, and high-dose dihydroquercetin + NLRP3 agonist group. The diopter and axial length of mice in each group were detected. The kit was used to detect the levels of superoxide dismutase(SOD)and malondialdehyde(MDA)in retinal tissue. RT-qPCR was used to detect the mRNA expressions of NLRP3, apoptosis-associated spot-like protein(ASC), Caspase-1, IL-1β and IL-18 in retinal tissues. Western blot was used to detect the expression of NLRP3, ASC, cleaved Caspase-1, IL-1β and IL-18 proteins in retinal tissues. TUNEL staining was used to detect apoptosis in retinal tissue.RESULTS: Compared with the control group, the diopter of the mice in the model group decreased, and axial length increased, and the SOD decreased whereas MDA, NLRP3, ASC, Caspase-1, IL-1β, IL-18 increased, and the rate of apoptosis in retinal tissue increased(all P<0.05). Compared with the model group, the diopter of mice in the low-, medium- and high-dose dihydroquercetin groups increased, axial length shortened, the SOD increased, whereas MDA, NLRP3, ASC, Caspase-1, IL-1β, IL-18 decreased, and the rate of apoptosis in retinal tissue decreased(all P<0.05). Compared with the high-dose dihydroquercetin group, the high-dose dihydroquercetin+NLRP3 agonist group had reduced diopter, increased axial length, decreased SOD levels, elevated MDA, NLRP3, ASC, Caspase-1, IL-1β, and IL-18 levels, as well as increased apoptosis rate in retinal tissue(all P<0.05).CONCLUSION: Dihydroquercetin can improve visual function in mice with form deprivation myopia by inhibiting pyroptosis and oxidative stress responses, which may be related to the suppression of NLRP3 inflammasome. NLRP3 agonists can partially mitigate the effects of high-dose dihydroquercetin on form deprivation myopia in mice.
