A case of special blood type with discrepancy between ABO genotype and serological results
10.13303/j.cjbt.issn.1004-549x.2026.01.018
- VernacularTitle:ABO基因型与血清学结果不符特殊血型1例
- Author:
Wenting JIA
1
;
Wei ZHANG
1
;
Limin CUI
1
Author Information
1. Inner Mongolia Autonomous Region Blood Center, Hohhot 010070, China
- Publication Type:Journal Article
- Keywords:
ABO subtype;
chimera;
blood transfusion safety;
gene sequencing
- From:
Chinese Journal of Blood Transfusion
2026;39(1):118-122
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To analyze the cause of discrepancy between ABO genotype B102/O01 and serological results in one case by PCR-SSP, to clarify the serological characteristics of this special blood group, and to explore relevant blood transfusion strategies. Methods: Blood group serological tests were performed on blood donor in August and December 2024, including forward and reverse ABO typing using tube method, H antigen identification, direct anti-human globulin test by tube method, red blood cell absorption-elution test, and determination of ABH blood group substance in saliva. Exons 1-7 of the ABO gene were amplified by PCR-SSP and sequenced. Results: The two separate serological tests consistently identified the donor as having an A
B phenotype, but the results of gene sequencing indicated a B102/O01 genotype, showing an discrepancy between serological and genetic results. Conclusion: It is very likely that the blood type of the blood donor is B102/O01 with a microchimerism of type A, or an AB type masked by A type reference gene.
