Methodological establishment of red blood cell lysis method for handling Rh typing double group samples
10.13303/j.cjbt.issn.1004-549x.2026.01.017
- VernacularTitle:红细胞裂解法处理Rh分型双群标本的方法学建立
- Author:
Lu LI
1
;
Bin WANG
2
;
Junjie WEI
1
;
Xiaolin SUN
1
;
Haiyun LIU
1
;
Weixin WU
1
;
Yinze ZHANG
1
Author Information
1. Shenzhen University General Hospital, Shenzhen 518055, China
2. Shenzhen Futian District Maternity & Child Healthcare Hospital, Shenzhen 518000, China
- Publication Type:Journal Article
- Keywords:
red blood cell lysis method;
Rh antigen typing;
double group;
Rh compatibility blood transfusion
- From:
Chinese Journal of Blood Transfusion
2026;39(1):114-117
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an accurate and rapid typing method for Rh typing of samples from patients who have received recent blood transfusions by utilizing the difference in osmotic fragility between fresh and old red blood cells. Methods: A lysing solution suitable for destroying old RBCs was prepared. Sixty-one samples collected in our hospital in 2024 with Rh typing of double groups were treated with the lysing solution to remove the old allogeneic red blood cells while preserving the patient's own fresh red blood cells, followed by repeat Rh typing tests. Results: For 61 samples with Rh typing in double groups, 41 were accurately detected identified through the red blood cell lysis method, yielding an identification rate of 67.21%. No significant difference was observed compared to the detection rate of the commonly used capillary centrifugation modified method (χ
=0.103, P>0.05). Conclusion: The red blood cell lysis method provides a novel and rapid experimental approach for clinical use in processing Rh-typed samples that are of double groups, thereby offering a basis for Rh compatibility blood transfusion.
