RBBP7 Promotes the Malignant Progression and Radioresistance of Esophageal Cancer through Hippo Signaling Pathway
10.12259/j.issn.2095-610X.S20251109
- VernacularTitle:RBBP7通过Hippo信号通路促进食管癌的恶性进展和放射抵抗性
- Author:
Zhiyu MA
1
;
Jie ZHENG
Author Information
1. 蚌埠医科大学附属皖北煤电集团总医院放疗一科,安徽 宿州 233400
- Keywords:
RBBP7;
Radiation resistance;
Esophageal cancer;
The Hippo signaling pathway
- From:
Journal of Kunming Medical University
2025;46(11):65-73
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of retinoblastoma-binding protein 7(RBBP7)in esophageal cancer and its molecular mechanism affecting its progression.Methods The tumor specimens and corresponding adjacent normal tissue specimens of 65 patients with esophageal cancer undergoing radical resection in the General Hospital of Wanbei Coal and Electricity Group Affiliated to Bengbu Medical University from January 2018 to December 2019 were collected.The expression of RBBP7 in esophageal cancer tissues was detected by immunohistochemistry,and the clinical correlation was analyzed.Eca109 and Kyse510 esophageal cancer cell lines were selected to detect the expression of RBBP7 in esophageal cancer cells by Western blot.The experiments were divided into Vector group,RBBP7 group,NC group and si-RBBP7 group by transfection technology.CCK-8 assay,colony formation assay,Transwell assay,wound healing assay and Western blot were used to detect the effect of RBBP7 on the proliferation,invasion,migration and radioresistance of esophageal cancer cells and its mechanism.Results The results of immunohistochemistry in clinical samples and Western blot in esophageal cancer cell lines showed that RBBP7 was highly expressed in esophageal cancer tissues compared with normal adjacent tissues(P<0.05),and esophageal cancer patients with high expression of RBBP7 had a poor prognosis(P<0.05).In the mechanism study,we found that overexpression of RBBP7 promoted the proliferation of esophageal cancer cells by CCK-8 and colony formation assay in vitro(P<0.05).Overexpression of RBBP7 promoted the invasion,migration,and radioresistance of esophageal cancer cells(P<0.05),and vice versa inhibited the proliferation,invasion,migration,and radioresistance of esophageal cancer cells(P<0.05).The results of Western blot showed that compared with the Vector control group,overexpression of RBBP7 may regulate the malignant progression and radioresistance of esophageal cancer by activating Hippo signaling pathway(P<0.05).Conclusion RBBP7 promotes the development and radioresistance of esophageal cancer through Hippo signaling pathway
