The Role of APOE in Drug Resistance of Colon Cancer Based on Bioinformatics and Cell Experiments
10.12259/j.issn.2095-610X.S20250902
- VernacularTitle:基于生物信息学和细胞实验探讨APOE在结肠癌耐药中的作用
- Author:
Ruo SHU
1
;
Huayou LUO
;
Lijun SONG
;
Yu GAO
;
Yan HOU
;
Xinfeng ZHANG
;
Ying LI
Author Information
1. 昆明医科大学第一附属医院胃肠与疝外科,云南 昆明 650032
- Keywords:
Colon cancer;
Chemotherapy resistance;
Apolipoprotein E;
Prognosis;
GEO
- From:
Journal of Kunming Medical University
2025;46(9):15-22
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the role and potential mechanism of apolipoprotein E(APOE)in drug resistance of colon cancer by bioinformatic tools and cellular experiments.Methods After downloading the microarray dataset GSE196900 from the GEO database,the online tool GEO2R was used to identify genes that were expressed differently in the drug-resistant and control groups.The differently expressed genes were then examined for Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment.The STRING database and Cytoscape software were used to build protein-protein interaction(PPI)networks and find hub genes.Hub genes'predictive significance in colon cancer was further assessed.Western blod and qRT-PCR were used to identify changes in APOE expression,whereas Transwell was used to identify changes in the colon cancer cells'capacity for invasion and migration.Results The analysis of GO and KEGG enrichment revealed that the differential genes derived from the GSE196900 dataset were primarily focused on receptor-ligand activity and cytokine-cytokine receptor interaction pathways.Using the CytoNCA plug-in in Cytoscape software,ten hub genes were obtained through PPI construction.Of these,the prognosis of the patients with colon cancer was negatively correlated with the expression of the APOE gene(P<0.05)and the overexpression of the APOE gene might significantly increase the migration and nvasivenessability of colon cancer cells(P<0.05).Conclusion The increased expression of APOE significantly promotes the migration and invasion ability of colon cancer cells,which may be one of the mechanisms by which APOE gene promotes tumor progression in the patients with colon cancer.
