Cyclin O Promotes the Proliferation and Metastasis of Cervical Cancer Cells by Regulating Glycolysis
10.12259/j.issn.2095-610X.S20250710
- VernacularTitle:细胞周期蛋白O通过调控糖酵解促进宫颈癌细胞增殖和转移
- Author:
Jingbo LIU
1
;
Yuan ZHANG
;
Mengjun LIU
;
Jing LIU
;
Hongli LIU
;
Jing ZHANG
Author Information
1. 蚌埠医科大学第一附属医院肿瘤妇科,安徽 蚌埠 233000
- Keywords:
CCNO;
Glycolysis and metabolism;
Cervical cancer;
PI3K/AKT signaling pathway
- From:
Journal of Kunming Medical University
2025;46(7):84-91
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of Cyclin O(CCNO)in cervical cancer and its molecular mechanism in the progression of cervical cancer.Methods The pathological sections of 60 patients with cervical cancer in the Department of Oncology and Gynecology of the First Affiliated Hospital of Bengbu Medical University from January 2018 to December 2023 were collected,and the expression of CCNO in cervical cancer was detected by immunohistochemistry.The cells were divided into Vector group,CCNO group,siNC group and siCCNO group by transfection technology.CCK-8 assay and colony formation assay were used to evaluate the ability of CCNO to affect cell proliferation.Transwell assay and wound healing assay were used to evaluate the effect of CCNO on cell invasion and migration.Glycolysis assay and Western blot assay were used to evaluate the effect and mechanism of CCNO on glycolysis of cervical cancer cells.Results The results of immunohistochemistry and WB showed that CCNO was highly expressed in cervical cancer(P<0.001).Overexpression of CCNO promoted the proliferation,invasion,migration and glycolysis of cervical cancer cells(P<0.05),whereas the opposite effect inhibited the proliferation,invasion,migration and glycolysis of cervical cancer cells(P<0.05).Bioinformatics analysis and WB results showed that CCNO overexpression may regulate the occurrence of cervical cancer by activating the PI3K/AKT signaling pathway(P<0.05).Conclusion Cyclin O may promote the proliferation and metastasis of cervical cancer cells by regulating glycolysis.
