EIF5A1 Promotes Proliferation,Migration and Invasion of Intrahepatic Cholangiocarcinoma Cells Through Wnt Signaling Pathway
10.12259/j.issn.2095-610X.S20250706
- VernacularTitle:EIF5A1通过Wnt/β-Catenin信号通路促进肝内胆管癌细胞增殖和迁移侵袭
- Author:
Shaohua YANG
1
;
Yongping XU
;
Zhuoyu ZHAO
;
Xingbao FANG
;
Zhenxing RUAN
Author Information
1. 曲靖市第一人民医院肝胆胰外科,云南 曲靖 655000
- Keywords:
Intrahepatic cholangiocarcinoma;
EIF5A1;
Wnt/β-Catenin signaling pathway;
Proliferation;
Migration and invasion
- From:
Journal of Kunming Medical University
2025;46(7):46-53
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of EIF5A1 in intrahepatic cholangiocarcinoma cell lines and human hepatobiliary duct epithelia,and its effect on the proliferation,migration and invasion ability and Wnt/β-Catenin signaling pathway in HUCCT1 cells.Methods Western blot was used to detect the basal expression level of EIF5A1 in intrahepatic cholangiocarcinoma cell lines and human intrahepatic cholangiocarcinoma epithelial cells.Transient transfection of siRNA was used to silence the expression of EIF5A1 in intrahepatic cholangiocarcinoma cell HUCCT1.The experimental groups were divided into blank control group(Con),siRNA1 group,and siRNA2 group.The most effective siRNA was screened by Western blot.The effects of EIF5A1 silencing on the proliferation,migration and invasion ability of HUCCT1 cells were detected by CCK-8,EdU cell proliferation assay and Transwell assay.The effect of EIF5A1 silencing on the Wnt/β-Catenin signaling pathway in HUCCT1 cells was detected by Western blot.Results The results of CCK-8 and EdU cell proliferation experiments showed that the proliferation ability of HUCCT1 cells decreased after EIF5A1 silencing(P<0.05),and Transwell migration and invasion experiments showed that the migration and invasion ability of Hucct1 cells decreased after EIF5A1 silencing(P<0.05).Western blot analysis revealed decreased expression of β-Catenin,Cyclin D1,MMP-2 and Survivin in Wnt/β-Catenin signaling pathway after EIF5A1 silencing(P<0.05).Conclusion EIF5A1 may promote the proliferation,migration and invasion of intrahepatic bile duct cancer cells through Wnt/β-Catenin signaling pathway.
