Effects and Mechanism of Quercetin on Osteogenic Differentiation of BMSCs
10.12259/j.issn.2095-610X.S20250504
- VernacularTitle:槲皮素对BMSCs成骨分化的影响及作用机制
- Author:
Jingxiao XU
1
;
Jia LIU
;
Shu YAO
;
Xi ZHANG
;
Jiang LI
;
Guiqin CUI
;
Xiaoling YI
;
Dongyun LI
Author Information
1. 昆明市中医医院药学部,云南 昆明 650000
- Keywords:
Quercetin;
Bone marrow mesenchymal stem cells;
Osteogenic differentiation;
Wnt/β-catenin
- From:
Journal of Kunming Medical University
2025;46(5):30-37
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect and mechanism of quercetin on osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs).Methods BMSCs were divided into a blank control group(Control)and quercetin(Quercetin)low-dose group(4.8 mL/kg),medium-dose group(9.6 mL/kg),and high-dose group(19.2 mL/kg)intervened with drug-containing serum,while the positive control group was treated with osteogenic differentiation medium,respectively.The cell cycle was analysed by flow cytometry,cell proliferation was detected by MTT assay,cell activity was determined by Alkaline phosphatase(ALP)assay kit,and calcified nodule formation was observed by alizarin red staining.The expression of β-catenin and the key factors of osteogenic differentiation,runt-related transcription factor 2(RUNX2)and osteocalcin(OCN),were detected by qPCR and Western blot,respectively.Results Compared with the control group,quercetin-containing serum significantly promoted the proliferation of BMSCs(P=0.000205,P=0.000063)and enhanced the formation of calcium nodules,and increased osteogenic and ALP activity after osteogenic differentiation.The results of qPCR and Western blot showed that the quercetin group significantly up-regulated the mRNA and protein expression of β-catenin(P<0.0001),RUNX2(P<0.0001)and OCN(P<0.0001)during osteogenic differentiation.Conclusion Quercetin can effectively promote the osteogenic differentiation of BMSCs,and its mechanism is achieved by activating the Wnt/β-catenin signaling pathway and up-regulating the expression of the osteogenesis-related transcription factor RUNX2.
