miR-21-5p Targetes STAT3 Reduce the OGD/R-induced Neuronal Injury
10.12259/j.issn.2095-610X.S20250502
- VernacularTitle:miR-21-5p靶向STAT3调节OGD/R诱导的神经元损伤
- Author:
Yanping LI
1
;
Qingyun LI
;
Rong HUO
;
Xiaolin DONG
;
Hongmei LI
;
Huan WEI
;
Yi ZENG
Author Information
1. 昆明医科大学附属延安医院神经内科,云南 昆明 650051
- Keywords:
Cerebral ischemia reperfusion injury;
miR-21-5p;
STAT3;
Oxygen-glucose deprivation/reoxygenation;
Apoptosis;
Neuroinflammation
- From:
Journal of Kunming Medical University
2025;46(5):12-20
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the potential mechanism of miR-21-5p in alleviating cerebral ischemia-reperfusion injury by targeting STAT3.Methods The HT22 cells were induced by OGD/R to construct a cell model of cerebral ischemia reperfusion injury.The expression of miR-21-5p was detected by RT-qPCR.The CCK-8 assay,TUNEL staining and flow cytometry were respectively used to detect the cell viability and apoptosis.ELISA assay was used to determine the contents of inflammatory factors IL-6,IL-10 and TNF-α in the cell supernatant.Western blot was used to detect the expression levels of p-STAT3/STAT3,Cleaved-Caspase-3,Bax and Bcl-2 proteins.The TargetScan database was used to predict the binding sites of miR-21-5p and STAT3.The dual-luciferase reporter gene assay was used to verify the targeting relationship between miR-21-5p and STAT3.Results The relative expression level of miR-21-5p was down-regulated in HT22 cells which induced by OGD/R(P<0.001).The cell viability(P<0.0001)was decreased and the apoptosis rate(P<0.001)was increased in OGD/R induced-HT22 cells.The contents of pro-inflammatory factors IL-6(P<0.001)and TNF-α(P<0.001)was increased,while the content of anti-inflammatory factor IL-10(P<0.001)decreased.After transfection with miR-21-5p mimic,cell viability was enhanced,apoptosis rate was reduced and neuroinflammation was inhibited.MiR-21-5p could target and bind to STAT3.After miR-21-5p inhibitor transfection,cell viability decreased,apoptosis was promoted,and neuroinflammation occurred;STAT3 inhibitor Stattic could reverse the effect of miR-21-5p inhibitor.Conclusion MiR-21-5p could specifically bind to STAT3 and reduce the neuroinflammation and apoptosis of OGD/R induced-HT22 cells.
