In Vitro Inhibition of Coxsackievirus by Blumea Balsamifera(L.)DC Extracts

VernacularTitle:艾纳香提取物体外抑制柯萨奇病毒的活性研究
Author: Huang LI ¹ ; Rongcheng WEN ; Li CHAI ; Xia LI ; Jinyan JIA ; Zhen CHEN
Author Information

1. 昆明医科大学康复学院,云南昆明 650500
Keywords: Blumea balsamifera(L.)DC.; Antiviral agents; Coxsackie virus
From: Journal of Kunming Medical University 2025;46(3):34-38
CountryChina
Language:Chinese
Abstract: Objective To investigate the in vitro antiviral effects of Blumea balsamifera(L.)DC.extract against Coxsackievirus B5(CVB5).Methods A series of dilutions of Coxsackievirus were prepared and co-cultured with RD cells to determine the TCID50 value.Subsequently,different concentrations of the extract were added to a 96-well plate containing RD cells to evaluate their impact on cell viability.The ability of Blumea balsamifera extract to inhibit Coxsackievirus was further observed in the 96-well plate containing RD cells and the extract.Results The TCID50 value of Coxsackie virus solution was 10-7.67.The inhibition rate of Blumea balsamifera extract against Coxsackievirus increased with concentration,with an IC50 value of 7.26 mg/L.At a concen-tration of 50 mg/L,the extract caused a decrease in RD cell viability(P＜0.05),but within the concentration range of 6.25 to 50 mg/L,it increased the viability of virus-infected RD cells(P＜0.05),with a selectivity index(SI)exceeding 6.89.Conclusion Blumea balsamifera(L.)DC.extract exhibits in vitro activity against Coxsackievirus.