Expression Characteristics and Clinical Pathological Features of TMEM33 in Lung Adenocarcinoma
10.12259/j.issn.2095-610X.S20250304
- VernacularTitle:TMEM33在肺腺癌的表达特征及临床病理特征
- Author:
Haijun LOU
1
;
Zhenyu ZHANG
;
Alimujiang SUREYAN
;
Menggen MENG
;
Hang CHEN
;
Muli WUDU
Author Information
1. 新疆医科大学基础医学院病理教研室,新疆乌鲁木齐 830017
- Keywords:
Lung adenocarcinoma;
TMEM33;
Biomarker;
Clinical pathological features;
Bioinformatics analysis
- From:
Journal of Kunming Medical University
2025;46(3):19-26
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study aimed to explore the expression pattern of transmembrane protein 33(TMEM33)in lung adenocarcinoma and its correlation with clinical pathological features.Methods Bioinformatics tools and public databases(e.g.,TCGA and GEO)were used to analyze TMEM33 expression data in lung cancer.Then,immunoblotting and real-time quantitative PCR were performed to detect TMEM33 protein and mRNA levels in four cell lines.Immunofluorescence and immunohistochemistry were also used to assess TMEM33 expression and localization in lung adenocarcinoma and normal lung tissue samples.Results Bioinformatics analysis revealed higher TMEM33 expression in lung adenocarcinoma than in normal lung tissue(P＜0.05)and a significant correlation between TMEM33 and SLC30A9 expression(P＜0.0001).Logistic regression analysis indicated an association between TMEM33 expression and tumor T stage(OR=0.48,P=0.044).Experimental results showed higher TMEM33 protein(P＜0.01)and mRNA(P＜0.001)levels in lung adenocarcinoma cell lines than in normal lung epithelial cells.Similarly,TMEM33 protein(P＜0.05)and mRNA(P＜0.01)levels were higher in lung adenocarcinoma tissues than in adjacent tissues.Immunohistochemistry confirmed high TMEM33 expression in lung adenocarcinoma tissues.Conclusion TMEM33 is highly expressed in lung adenocarcinoma,associated with malignancy and T stage,and may be a potential prognostic and therapeutic target.
