Preparation of Graphene Oxide Nanosheets and Their Portable Colorimetric Determination of Biothiols
10.12259/j.issn.2095-610X.S20250205
- VernacularTitle:氧化石墨烯纳米片的制备及其对生物硫醇的便携式比色测定
- Author:
Xin WANG
1
;
Yubo WEI
;
Yupeng LI
;
Jiacan YAO
;
Fang YU
Author Information
1. 昆明医科大学药学院暨云南省天然药物药理重点实验室,云南 昆明 650500;昆明医科大学现代生物医药产业学院,云南 昆明 650500
- Keywords:
Graphene oxide;
Biothiols;
Colorimetric sensing;
Peroxide mimics enzymes
- From:
Journal of Kunming Medical University
2025;46(2):30-36
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a method capable of real-time,rapid,and visual detection of MEs,providing a powerful tool for early diagnosis and assessment of diseases.Methods In this study,flake graphene oxide(2D GO)with excellent peroxidase-like activity(POD-like)was prepared by a modified Hummers'method,and a sensing strategy for the rapid visual detection of MEs was constructed by integrating colorimetry with a smartphone.Results 2D GO can catalyse the decomposition of hydrogen peroxide(H2O2)to produce highly oxidative hydroxyl radicals(·OH),which oxidize the colourless 3,3',5,5'-tetramethylbenzidine(TMB)into blue ox-TMB.The strong reducing effect of MEs on the"2D GO+TMB+H2O2"colorimetric sensing system enables rapid visual detection of MEs.Conclusion The colorimetric platform constructed based on 2D GO has a wide linear detection range(10-1 000 μmol/L)and a good detection limit(LOD<7 μM),and was successfully used for the determination of MEs in foetal bovine serum samples,with satisfactory recovery rates.
