Study on the levels and potential mechanisms of degranulated basophils in the blood of patients with sepsis
10.3760/cma.j.cn114656-20250426-00324
- VernacularTitle:脓毒症患者血液嗜碱性粒细胞的脱颗粒水平及其潜在机制研究
- Author:
Yuhan SUN
1
;
Shengyong REN
;
Mengmeng ZHAN
;
Xin DONG
;
Shihao LIU
;
Congyi ZHAO
;
Junling WANG
;
Bingyu QIN
Author Information
1. 郑州大学人民医院河南省人民医院重症医学部，郑州 450003
- Keywords:
Sepsis;
Peripheral blood mononuclear cell population;
Granulocyte population;
Basophils;
Degranulation;
IL-9;
Potential mechanism
- From:
Chinese Journal of Emergency Medicine
2025;34(10):1373-1381
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the degranulation levels of basophils in peripheral blood mononuclear cell (PBMC) and granulocyte populations between healthy subjects and patients with sepsis, and to explore the underlying mechanisms. Additionally, plasma cytokine levels were measured in these volunteers.Methods:Peripheral blood samples were collected from both healthy individuals and sepsis patients. The degranulation level of basophils in sepsis patients and its potential mechanisms were examined. Plasma levels of IL-1β, IL-9, and IL-10 were detected, and Pearson correlation analysis was performed to assess the relationship between degranulated basophils in the granulocyte population and IL-9 levels.Results:Compared with healthy subjects, sepsis patients showed a reduction in basophil percentages within PBMC and granulocyte populations by 94.8% and 37.9%, respectively ( Z = -6.441, P < 0.05; Z = -2.681, P < 0.05). In contrast, both the proportion and number of degranulated basophils in the granulocyte population were increased (both P < 0.05). Plasma levels of IL-1β, IL-9, and IL-10 were significantly elevated in sepsis patients--by 80.6%, 36.7%, and 11.9-fold, respectively ( Z = -4.258, P < 0.05; Z = -3.606, P < 0.05; Z = -4.814, P < 0.05). Moreover, plasma IL-9 levels were highly correlated with both the percentage and count of degranulated basophils in the granulocyte population (both P < 0.05). GO and KEGG enrichment analyses revealed cytological changes and potential mechanisms involving basophils in the PBMC of sepsis patients. Conclusions:The decreased total count of basophils in sepsis patients may elevate the risk of secondary infection. Degranulated basophils in the granulocyte population may contribute to excessive inflammatory responses through IL-9 secretion.
