The characteristics of plasma lipids in silicosis rat models were studied based on lipid metabolomics
10.3760/cma.j.cn114656-20241203-00835
- VernacularTitle:基于脂质代谢组学研究急性肺损伤大鼠模型中肺组织脂质特征
- Author:
Chen WANG
1
;
Yuhua ZHANG
;
Yongpeng XIE
;
Xiaobing CHEN
;
Xiaomin LI
Author Information
1. 南京医科大学连云港临床医学院连云港市第一人民医院急诊科，连云港 222006
- Keywords:
Acute lung injury;
Acute respiratory distress syndrome;
Lipidomics;
Lipopolysaccharide;
Phosphatidylcholines;
Lysophosphatidylcholines;
Glycerophospholipid
- From:
Chinese Journal of Emergency Medicine
2025;34(8):1064-1070
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate differentially expressed lipid molecules and their associated metabolic pathways in lung tissue using lipidomic analysis in a rat model of acute lung injury (ALI).Methods:An ALI rat model was established via intratracheal instillation of lipopolysaccharide (LPS). Twenty rats were randomly allocated into an ALI group and a control group ( n = 10 per group). The left lung was subjected to histopathological evaluation, while the right lung underwent untargeted lipidomics analysis. Ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was employed for lipid profiling. Principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) were performed to assess intergroup differences and determine variable importance in projection (VIP) scores. Differential lipids were screened based on VIP and fold change.Lipid identification and metabolic pathway analysis were conducted using MetaboAnalyst 5.0 and the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. Results:Among 1 022 detected lipid molecules, 47 were differentially expressed (VIP > 1, FC > 2.0 or < 0.5, P< 0.05). Subsequent analysis identified 12 structurally annotated lipids ( P < 0.05), predominantly enriched in glycerophospholipid metabolism, linoleic acid metabolism, and α-linolenic acid metabolism pathways. Notably, phosphatidylcholines (PCs) and lysophosphatidylcholines (LPCs) exhibited significant alterations in the ALI group. Conclusions:The ALI model demonstrated substantial dysregulation of lipid metabolism, particularly involving PCs and LPCs, with prominent perturbations in glycerophospholipid metabolism. This provides a scienctific basis for indepth research on the pathogenesis mechanism of ALI/acute respiratory distress syndrome (ARDS) .
