Plasma proteomic study of patients with acute diquat poisoning based on nano-liquid chromatography tandem quadrupole time-of-flight mass spectrometry
10.3760/cma.j.issn.1671-0282.2025.03.010
- VernacularTitle:基于纳升液相色谱串联四极杆飞行时间质谱的急性敌草快中毒患者的血浆蛋白质组学研究
- Author:
Tianqi ZHANG
1
;
Na YANG
;
Min WANG
;
Xuemei LUO
;
Huaijun ZHU
Author Information
1. 南京鼓楼医院（南京大学医学院附属鼓楼医院）药学部，南京 210008
- Keywords:
Diquat;
Nanoliter liquid chromatography tandem quadrupole time-of-flight mass spectrometry;
Proteomics;
Prognosis;
Biomarker
- From:
Chinese Journal of Emergency Medicine
2025;34(3):342-349
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression characteristics of plasma proteins in patients with acute diquat poisoning by proteomic technology, and to screen the targets and biomarkers related to the diagnosis, treatment and prognosis of acute diquat poisoning.Methods:This study was a retrospective case-control study. Patients with diquat poisoning admitted to Nanjing University Affiliated Drum Tower Hospital from August 2022 to December 2023 were enrolled. Healthy volunteers matched for age and gender during the same period were selected as the control group. The general clinical data and plasma samples were collected, and qualitative and quantitative analyses of plasma proteins were performed by proteomic technology based on nanoliter liquid chromatography tandem quadrupole time-of-flight mass spectrometry. Mann-Whitney U test was used to compare differences of plasma protein expression between the two groups. Results:A total of 8 diquat poisoning patients and 7 healthy individuals were included in this study, and 491 plasma proteins were identified. Compared with control group, 76 differentially expressed proteins were obtained from the poisoned group, including 35 up-regulated proteins and 41 down-regulated proteins. GO analysis showed that the differentially expressed proteins were mainly associated with oxygen transporter activity, receptor binding, and hyaluronic acid-binding endopeptidase inhibitor activity. KEGG pathway enrichment analysis revealed that differentially expressed proteins were associated with complement and coagulation cascades, neutrophil extracellular trap formation, and platelet activation. In addition, the expression of adiponectin (ADIPOQ) was not only increased in poisoned patients, but also significantly higher in non-survivors than in survivors ( P<0.01). Conclusions:Multiple plasma proteins with abnormal expression may be involved in the pathological process of acute diquat poisoning. ADIPOQ may have potential application value in the diagnosis, treatment, and prognosis of acute diquat poisoning.
