Study on in vitro and in rat’s vivo evaluation methods of quetiapine adsorption by activated charcoal
10.3760/cma.j.issn.1671-0282.2025.03.009
- VernacularTitle:活性炭对喹硫平吸附效果的体外和大鼠体内评价方法研究
- Author:
Gen BA
1
;
Decai CAO
;
Qifang SHI
;
Weiran XIE
;
Beiyan ZHANG
;
Hao SUN
;
Zhengsheng MAO
;
Jinsong ZHANG
Author Information
1. 南京医科大学中毒研究所，南京 210029
- Keywords:
Activated charcoal;
Acute poisoning;
Quetiapine;
Pharmacokinetics;
Functional modeling
- From:
Chinese Journal of Emergency Medicine
2025;34(3):335-341
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the impact of various conditions on the adsorption of quetiapine by activated carbon, establish a method for evaluating the adsorption efficacy of activated carbon on quetiapine, and assess the adsorption effects both in vitro and in vivo.Methods:In vitro experiments involved incubating activated carbon with quetiapine under different conditions, including varying organic solvent contents, types of organic solvents, adsorption temperatures, adsorption times, and pH. After reaching equilibrium, the mixtures were centrifuged, and the supernatants were collected. The concentration of quetiapine in the supernatants was measured using LC-MS/MS, and the adsorption rates were calculated. The log-transformed concentration of activated carbon was used as the independent variable and the adsorption rate as the dependent variable for function fitting using Origin 2021 software. In the in vivo experiments, rats were administered quetiapine orally, followed by 125 mg/mL of activated carbon in the experimental group. Blood samples were collected at multiple time points pre- and post-administration (0.17 h, 0.33 h, 0.50 h, 0.75 h, 1 h, 1.5 h, 2 h, 4 h, 6 h, 12 h, and 24 h). Plasma samples were pre-treated and the quetiapine concentrations were determined using LC-MS/MS. Pharmacokinetic parameters for both control and experimental groups were calculated using DAS 2.0 software.Results:The factors such as organic solvent content, type of organic solvent, adsorption temperature, adsorption time, and pH value significantly influenced the adsorption efficiency of quetiapine by activated carbon, leading to the optimization and standardization of the in vitro adsorption methodology. Among the 100 different adsorption function models tested, the Boltzmann function was identified as the most suitable models for describing the adsorption of quetiapine by activated carbon. Pharmacokinetic analysis showed that the experimental group treated with activated carbon exhibited significantly reduced C max and AUC for quetiapine compared to the control group. Conclusion:The results of both in vitro and in vivo experiments demonstrate that activated carbon effectively adsorbs quetiapine, providing a potential method for mitigating quetiapine absorption.
