Properties,functions,and classification of linkers in molecular design of fusion proteins
10.7644/j.issn.1674-9960.2025.09.009
- VernacularTitle:融合蛋白分子设计中连接子的性质、功能和分类
- Author:
Tiantian YANG
1
;
Yuan WANG
;
Min LI
;
Guangyu ZHAO
Author Information
1. 牡丹江医学院公共卫生学院,黑龙江牡丹江 157011;军事科学院军事医学研究院病原微生物生物安全全国重点实验室,北京 100071
- Keywords:
linker;
protein;
function
- From:
Military Medical Sciences
2025;49(9):700-706
- CountryChina
- Language:Chinese
-
Abstract:
In the era of rational molecular design of fusion proteins,linker selection has garnered significant attention as a critical determinant of construct functionality.Suboptimal selection of linkers may result in such structural perturbations as protein misfolding,reduced expression yields,and compromised bioactivity.Consequently,the strategic selection of linkers tailored to specific objectives of molecular design by optimizing spatial orientation,maintaining domain autonomy or enabling post-translational modifications has emerged as a pivotal research frontier.Given these challenges,this review outlines the common properties of linkers,ways of classification,and the functional-structural interplay in current applications.Furthermore,we propose context-dependent selection frameworks for therapeutic proteins,biosensors,and enzyme cascades,which can serve as a systematic methodology to guide linker optimization in next-generation fusion protein engineering.
