RPA-CRISPR/Cas12a-based rapid detection of Clostridium botulinum in food
10.7644/j.issn.1674-9960.2025.09.005
- VernacularTitle:基于RPA-CRISPR/Cas12a的食品中肉毒杆菌快速检测方法建立
- Author:
Zishu LAN
1
;
Shuqing ZHOU
;
Tianjiao CHEN
;
Rong TAN
;
Min JIN
;
Dong YANG
Author Information
1. 军事科学院军事医学研究院,天津 300050
- Keywords:
food;
Clostridium botulinum;
CRISPR-Cas12a;
rapid detection
- From:
Military Medical Sciences
2025;49(9):674-680
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a rapid detection method for Clostridium botulinum in food.Methods A rapid detection method based on RPA-CRISPR/Cas12a was developed by integrating recombinase polymerase amplification(RPA)with the CRISPR-Cas12a system.After the reaction conditions were optimized,the method's sensitivity,specificity,and usefulness were methodically confirmed.Results and Conclusion The optimized method achieved detection within 1 hour,with a limit of detection(LOD)of 1.91 copies/μL.No cross-reactivity was observed with non-target pathogens.The RPA-CRISPR/Cas12a-based detection method developed in this study exhibits high specificity,sensitivity,and operational simplicity and may provide a feasible solution for the rapid detection of foodborne pathogens.
