Effects of myeloid cell-specific knockout of G-CSFR on the progression of acute radiation pneumonitis in mice
10.7644/j.issn.1674-9960.2025.08.004
- VernacularTitle:髓系细胞特异性敲除G-CSFR对小鼠急性放射性肺炎进程的影响研究
- Author:
Zhe YANG
1
;
Min DUAN
;
Yumeng YE
;
Yongyi WANG
;
Jiao ZHANG
;
Xuejia WANG
;
Jun WANG
;
Yang LI
Author Information
1. 承德医学院基础医学院,河北承德 067000;军事科学院军事医学研究院,北京 100850
- Keywords:
ionizing radiation;
mice;
radiation-induced pneumonia;
granulocyte colony-stimulating factor;
neutrophils
- From:
Military Medical Sciences
2025;49(8):582-588
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the impact of myeloid cell-specific knockout of the granulocyte colony-stimulating factor receptor(G-CSFR)on the progression of acute radiation pneumonitis.Methods Myeloid cell-specific G-CSFR knockout(G-CSFR-/-,Lyz2-cre)mice were constructed.G-CSFR-/-,Lyz2-cre and C57BL/6N mice underwent a single whole-body irradiation with 6.5 Gy of 60Co γ-rays to establish a model of radiation injury.The lung function of mice was assessed using a mouse lung function test system at 3,7 and 14-days post γ-ray irradiation.Pathological changes in the lung tissue were analyzed via hematoxylin and eosin(HE)staining of paraffin sections.Tumor necrosis factor-α(TNF-α)and interleukin-10(IL-10)levels were measured via radioimmunoassay.IL-8 and its receptor CXCR2 were quantified using enzyme-linked immunosorbent assay(ELISA).The infiltration of neutrophils in lung tissue was evaluated by immunohistochemical detection of myeloperoxidase.Results At 3-,7-and 14-days post-irradiation with 6.5 Gy of 60Co γ-rays,there were no significant differences observed in lung function or interstitial inflammatory lesions between G-CSFR-/-,Lyz2-cre mice and C57BL/6N mice.However,the infiltration of neutrophils in lung tissue of G-CSFR-/-,Lyz2-cre mice was significantly reduced(P＜0.01),and the levels of IL-8,CXCR2 and TNF-α in lung tissues were markedly lower than in C57BL/6N mice(P＜0.05).Conclusion The myeloid cell-specific knockout of G-CSFR can effectively diminish neutrophil infiltration as well as inflammatory cytokine levels in lung tissues following radiation exposure.
