Screening of pyroptosis-related genes in pulmonary plague and molecular mechanisms based on bioinformatics analysis
10.7644/j.issn.1674-9960.2025.04.006
- VernacularTitle:基于生物信息学分析的肺鼠疫细胞焦亡相关基因筛选及分子机制探索
- Author:
Hao XIE
1
;
Lingli AI
;
Duo SU
;
Lu LI
;
Lingfei HU
;
Dongsheng ZHOU
;
Bo GAO
;
Huiying YANG
Author Information
1. 军事科学院军事医学研究院,北京 100071
- Keywords:
pneumonic plague;
Yersinia pestis;
bioinformatics;
pyroptosis;
cysteinyl aspartate-specific proteinases-4
- From:
Military Medical Sciences
2025;49(4):281-288
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the biological functions of pyroptosis-related genes in pneumonic plague using bioinformatics methods,and to evaluate their potential applicability as diagnostic markers.Methods The pneumonic plague-related dataset GSE220123 was retrieved from the Gene Expression Omnibus(GEO)database and screened for differentially expressed pyroptosis-related genes(DE-PRGs).The functions of DE-PRGs were studied via Gene Ontology(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses,and immune infiltration analysis.The hub genes were identified via protein-protein interaction(PPI)network analysis,and further screened for key genes with sustained high expression characteristics based on differential expression analysis.The relative expression levels of the key genes were verified using the reverse transcription real-time quantitative PCR(qPCR)method.Results A total of 17 DE-PRGs were screened,and PPI network analysis revealed 7 Hub genes.Among them,Casp4 continued to be up-regulated during the course of pneumonic plague.The results of reverse transcription qPCR were consistent with the those of bioinformatic analyses.Conclusion DE-PRGs play a crucial role in the immune response of pneumonic plague,especially Casp4,which has significant applications as a diagnostic biomarker and potential therapeutic target for pneumonic plague.
