Involvement of piriform cortex in 5-choice serial reaction time task(5-CSRTT)attention behavior in mice
10.7644/j.issn.1674-9960.2025.03.004
- VernacularTitle:小鼠梨状皮质在五项选择连续反应时间任务(5-CSRTT)注意行为中的功能研究
- Author:
Mingze CAI
1
;
Hao LI
;
Yan YU
;
Wei SHEN
;
Haitao WU
Author Information
1. 青岛大学基础医学院,山东青岛 266071
- Keywords:
attention;
5-choice serial reaction time task(5-CSRTT);
piriform cortex;
c-Fos;
chemogenetics
- From:
Military Medical Sciences
2025;49(3):185-191
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of the piriformcortex in regulating attentional behavior in mice.Methods Adult male C57BL/6J mice were subjected to a 5-choice serial reaction time task(5-CSRTT).Immunofluorescence staining was performed to detect expressions of cellular FBJ murine osteosarcoma viral oncogene homolog protein(c-Fos)in the piriform cortex.The changes of attentional behavior in the 5-CSRTT test were explored following either stereotactic injection of diphtheria toxin A virus to specifically damage piriform cortex neurons or chemogenetical inhibition of the neuronal activity inthe piriform cortex.Results It was found that the expression of c-Fos in the 5-CSRTT-tested mice was significantly increased compared to the control.Both lesion and chemogenetic inhibition of piriform cortex neuronsreduced the accuracy of attention,but omission rates and premature responses remained unaffected in the 5-CSRTT test.Conclusion Piriform cortex neuronsmay play a critical role in modulating attentional processes in mice.
