Mechanism of action of Guizhi Fuling Pill in treating chronic prostatitis based on network pharmacology and molecular docking
- VernacularTitle:基于网络药理学和分子对接探讨桂枝茯苓丸治疗慢性前列腺炎的作用机制
- Author:
Ji SUN
1
;
Xinfeng XIA
;
Peng JIN
;
Wei ZHONG
;
Yanlin ZHAO
;
Qinglei HANG
;
Guohui ZHU
Author Information
- Keywords: Guizhi Fuling Pill; chronic prostatitis; network pharmacology; molecular doc-king; signal pathway; target; gene ontology; Kyoto Encyclopedia of Genes and Genomes
- From: Journal of Clinical Medicine in Practice 2025;29(20):72-77
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the mechanism of action of Guizhi Fuling pill in treating chro-nic prostatitis(CP)using network pharmacology and molecular docking techniques.Methods Compo-nents of Guizhi Fuling pill were collected from the Traditional Chinese Medicines Systems Pharmacolo-gy Platform(TCMSP),and target information was obtained from the SwissTarget database.Targets for chronic prostatitis were screened from the GeneCards,OMIM,CTD,and DisGeNET disease data-bases.A protein-protein interaction(PPI)network was established and analyzed.Gene ontology(GO)functional annotation and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway en-richment analysis were performed using the DAVID database.The Cytoscape software was employed to construct an association network linking the components of Guizhi Fuling Pill,their targets,and the targets of chronic prostatitis.Molecular docking was conducted using AutoDock Vina software to verify the binding stability between the components of Guizhi Fuling pill and their targets.Results After screening and deduplication in the TCMSP database,76 components of Guizhi Fuling Pill were iden-tified,and 655 component targets were retrieved from the SwissTarget database.There were 190 intersecting targets between GuizhiFuling Pill and chronic prostatitis.GO analysis indicated that Guizhi Fuling Pill may treat chronic prostatitis by participating in processes such asapoptosis,ATP binding,and signal transduction.KEGG analysis suggested that Guizhi Fuling Pill can regulate pathways such as phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)and mitogen-acti-vated protein kinase(MAPK)to intervene in chronic prostatitis.Molecular docking data demonstra-ted that the components of Guizhi Fuling pill exhibited stable conformations with their targets.Con-clusion The components of Guizhi Fuling Pill can stably bind to their targets and exert therapeutic effects on chronic prostatitis through multiple targets and pathways.
