Correlations of expression levels of microRNA-147,Ki-67 and carcinoembryonic antigen with lymph node metastasis in invasive pulmonary adenocarcinoma
- VernacularTitle:微小RNA-147、Ki-67及癌胚抗原表达水平与浸润性肺腺癌淋巴结转移的关系
- Author:
Panpan ZHU
1
;
Xiaoping YANG
Author Information
- Keywords: microRNA-147; Ki-67; carcinoembryonic antigen; invasive pulmonary adeno-carcinoma; lymph node metastasis; receiver operating characteristic curve; immunohistochemistry; TNM staging
- From: Journal of Clinical Medicine in Practice 2025;29(14):7-12
- CountryChina
- Language:Chinese
- Abstract: Objective To explore correlations of expression levels of microRNA-147(miR-147),Ki-67 and carcinoembryonic antigen(CEA)with lymph node metastasis of invasive lung adenocarci-noma.Methods A total of 160 patients with invasive pulmonary adenocarcinoma were selected as study subjects.Based on presence of lymph node metastasis,patients were divided into lymph node metastasis group(n=74)and non-lymph node metastasis group(n=86).Immunohistochemistry was employed to detect the expression levels of miR-147 and Ki-67 in cancer tissues.The serum CEA level was measured using the chemiluminescence method.The correlations of the expression levels of miR-147,Ki-67 and serum CEA with the clinicopathological characteristics of patients were analyzed.The value of miR-147,Ki-67 and CEA in predicting lymph node metastasis was evaluated using the receiver operating characteristic(ROC)curve.Results The expression level of miR-147 in the lymph node metastasis group was significantly lower than that in the non-lymph node metastasis group,while the expression levels of Ki-67 and serum CEA were significantly higher in the lymph node me-tastasis group than in the non-lymph node metastasis group(P＜0.05).Patients with a tumor diameter≥3 cm or TNM stage Ⅲ to Ⅳ had significantly lower miR-147 expression levels in cancer tissues compared to those with a tumor diameter＜3 cm or TNM stage Ⅰ to Ⅱ(P＜0.05).The Ki-67 ex-pression level in cancer tissues was significantly higher in patients with a tumor diameter ≥3 cm than in those with a tumor diameter＜3 cm(P＜0.05).The serum CEA level was significantly higher in patients with TNM stage Ⅲ to Ⅳ than in those with TNM stage Ⅰ to Ⅱ(P＜0.05).The expres-sion level of miR-147 was negatively correlated with Ki-67,serum CEA and lymph node metastasis(P＜0.05);Ki-67 and serum CEA were positively correlated with lymph node metastasis(P＜0.05);Ki-67 was positively correlated with serum CEA(P＜0.05).The areas under the ROC curves for miR-147,Ki-67 and CEA in predicting lymph node metastasis were 0.785,0.835 and 0.798,respectively(P＜0.05).Conclusion The expression levels of miR-147 and Ki-67 in cancer tissues and the serum CEA level are correlated with lymph node metastasis in invasive pulmo-nary adenocarcinoma.
