Role of mitochondrial autophagy-related genes in colorectal cancer based on spatial transcriptomics and single-cell sequencing analysis
- VernacularTitle:基于空间转录组和单细胞测序分析线粒体自噬相关基因在结肠癌中的作用
- Author:
Changxin SHU
1
;
Chen WEI
;
Dong TANG
Author Information
- Keywords: colorectal cancer; single-cell sequencing; spatial transcriptomics; mitochondrial autophagy; machine learning algorithms; tumor microenvironment; RPS5 gene; fibroblasts
- From: Journal of Clinical Medicine in Practice 2025;29(7):92-99
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the role of mitochondrial autophagy-related genes in color-ectal cancer using machine learning algorithms to analyze single-cell sequencing data and spatial tran-scriptomics data.Methods Key mitochondrial autophagy-related genes were screened using Support Vector Machine-Recursive Feature Elimination(SVM-RFE)and Random Forest algorithms.Compre-hensive assessments of cell-cell interactions,pseudo-time analysis,and gene spatial localization were conducted by integrating single-cell sequencing data and spatial transcriptomics data.Knockdown ex-periments were performed on the identified core genes to verify their effects on the proliferation,mi-gration,and invasion capabilities of colorectal cancer cells.Results RPS5 was identified as a core gene in the progression of colorectal cancer.RPS5-positive epithelial cells were primarily located in the tumor core region and interacted with fibroblasts.Immunohistochemical experiments demonstrated that RPS5 expression was significantly higher in colorectal cancer tissues compared to adjacent tis-sues.Functional experiments showed that knockdown of RPS5 significantly inhibited the proliferation,migration,and invasion capabilities of colorectal cancer cells.Conclusion RPS5 is a key molecule promoting the progression of colorectal cancer,which provides a new potential target for precision therapy in colorectal cancer.
