Effect of lncRNA JHDM1D-AS1 targeting microRNA-421 on hydrogen peroxide induced oxidative damage of cardiomyocytes
- VernacularTitle:长链非编码RNA组蛋白去甲基化酶1同系物D反义RNA1靶向微小RNA-421对过氧化氢诱导的心肌细胞氧化损伤的影响
- Author:
Shuai WANG
1
;
Shen WU
;
Yang GU
Author Information
- Keywords: long non-coding RNA; Jumonji C domain containing histone demethylase 1 hom-olog D antisense RNA1; microRNA-421; hydrogen peroxide; cardiomyocytes; apoptosis; oxidative stress response
- From: Journal of Clinical Medicine in Practice 2025;29(5):88-94
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the effect of long non-coding RNA Jumonji C domain con-taining histone demethylase 1 homolog D antisense RNA1(lncRNA JHDM1D-AS1)targeting microR-NA-421(miR-421)on hydrogen peroxide(H2O2)induced oxidative damage in cardiomyocytes(H9C2 cells).Methods H9C2 cells were divided into control(Con)group,H2O2 group,H2O2+pcDNA group,H2O2+pcDNA-JHDM1D-AS1 group,H2O2+anti-miR-NC group,H2O2+anti-miR-421 group,H2O2+pcDNA-JHDM1D-AS1+miR-NC group,and H2O2+pcDNA-JHDM1D-AS1+miR-421 group.The expressions of JHDM1D-AS1 and mi R-421 were detected by real-time fluorescent quantitative PCR(RT-qPCR).The superoxide dismutase(SOD)activity,malondialdehyde(MDA)level,and lactate dehydrogenase(LDH)level in the culture medium of H9C2 cells were measured by colorimetry.Flow cytometry was used to evaluate the apoptosis rate of H9C2 cells.The targeting rela-tionship between JHDM1D-AS1 and mi R-421 was verified by dual luciferase reporter gene assay.Re-sults Compared with the Con group,the H2O2 group showed decreased levels of JHDM1D-AS1 and SOD activity,and increased levels of MDA,LDH,apoptosis rate,and miR-421 in H9C2 cells,with significant between-group differences(P＜0.05).Compared with the H2O2+pcDNA group,the H2O2+pcDNA-JHDM1D-AS1 group exhibited increased SOD activity and decreased miR-421 ex-pression level,MDA level,LDH level,and apoptosis rate in H9C2 cells,with significant between-group differences(P＜0.05).Compared with the H2O2+anti-miR-NC group,the H2O2+anti-miR-421 group showed increased SOD activity and decreased MDA level,LDH level,and apoptosis rate in H9C2 cells,with significant between-group differences(P＜0.05).MiR-421 was identified as a target gene of JHDM1D-AS1.Compared with the H2O2+pcDNA-JHDM1D-AS1+miR-NC group,the H2O2+pcDNA-JHDM1D-AS1+miR-421 group exhibited decreased SOD activity and in-creased MDA level,LDH level,and apoptosis rate in H9C2 cells,with significant between-group differences(P＜0.05).Conclusion LncRNA JHDM1D-AS1 inhibits apoptosis and oxidative stress by targeting and downregulating miR-421 expression,thereby alleviating H2O2-induced oxida-tive damage in cardiomyocytes.
