Downregulation of microRNA-550a-5p targetedly regulates scavenger receptor class A member 3 to inhibit progression of non-small cell lung cancer
- VernacularTitle:下调微小RNA-550a-5p靶向调控清道夫受体A类成员3抑制非小细胞肺癌进展
- Author:
Juan XU
1
;
Ruru ZHANG
;
Feiwen YU
;
Xiangfeng HE
;
Mengqing PENG
Author Information
- Keywords: non-small cell lung cancer; microRNA-550a-5p; scavenger receptor class A member 3; proliferation; migration; invasion
- From: Journal of Clinical Medicine in Practice 2025;29(3):22-29
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the effects of downregulating microRNA(miR)-550a-5p on the proliferation,migration and invasion of non-small cell lung cancer(NSCLC)and to explore its mo-lecular mechanisms.Methods The miR-550a-5p inhibitor and its negative control(inhibitor NC),as well as small interfering RNA targeting scavenger receptor class A member 3(si-SCARA3)and its negative control(si-NC)were individually or co-transfected into A549 cells.These cells were desig-nated as inhibitor NC group,miR-550a-5p inhibitor group,miR-550a-5p inhibitor+si-NC(co-trans-fection)group and miR-550a-5p inhibitor+si-SCARA3(co-transfection)group.Cell proliferation was assessed by CCK-8 assay;colony formation ability was evaluated using a plate cloning method;cell migration and invasion were detected by scratch assay and Transwell chamber assay,respectively;levels of matrix metalloproteinase(MMP)-2 and MMP-9 in the supernatant of A549 cells were measured by enzyme-linked immunosorbent assay(ELISA);apoptosis levels were determined by Annexin V/PI double staining.Results Compared with adjacent normal tissues,the expression level of miR-550a-5p was increased,while SCARA3 mRNA expression was decreased in NSCLC tissues(P＜0.05).Dual-luciferase reporter assays confirmed that miR-550a-5p directly targeted SCARA3.Compared with the inhibitor NC group,cell proliferation rate,colony formation number,migration and invasion ability of the miR-550a-5p inhibitor group decreased,apoptosis rate increased(P＜0.05).Compared with the inhibitor NC group,the expression levels of MMP-2 and MMP-9 in cell supernatant of the miR-550a-5p inhibitor group were decreased,and the expression levels of SCARA3 protein were increased(P＜0.05).Compared with the miR-550a-5p inhibitor group or the miR-550a-5p inhibitor+si-NC group,cell proliferation rate,clonal colony formation number,migration and invasion ability of the miR-550a-5p inhibitor+si-SCARA3 group were increased,and apoptosis rate was decreased(P＜0.05).Compared with the miR-550a-5p inhibitor group or the miR-550a-5p inhibitor+si-NC group,the miR-550a-5p inhibitor+si-SCARA3 group showed in-creased expression levels of MMP-2 and MMP-9 in the cell supernatant and decreased SCARA3 pro-tein expression levels(P＜0.05).Conclusion Downregulation of miR-550a-5p inhibits the prolif-eration,migration and invasion of NSCLC cells,and promotes apoptosis,possibly through upregulat-ing SC AR A3 expression.
