Impact and mechanism of NOD-like receptor thermal protein domain-associated protein 3 on pathological features and inflammatory response in a rat model of hemorrhoids
- VernacularTitle:NOD样受体热蛋白结构域相关蛋白3对痔疮模型大鼠创面病理特征和炎症反应的影响及机制研究
- Author:
WUMAIERJIANG AISHANJIANG
1
;
AIMAITI ADILIJIANG
;
Xuan WU
;
WUBULIHASIMU GULINIGEER
;
Shu YANG
Author Information
- Keywords: hemorrhoids; NOD-like receptor thermal protein domain-associated protein 3; cyclic adenosine monophosphate; transient receptor potential vanilloid 1; inflammatory factors
- From: Journal of Clinical Medicine in Practice 2025;29(2):102-107
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the effects of NOD-like receptor thermal protein domain-associated protein 3(NLRP3)on wound pathological characteristics,inflammatory responses,and cyclic adenosine monophosphate(cAMP)/transient receptor potential vanilloid subtype 1(TRPV1)signaling pathways in a rat model of hemorrhoids.Methods Forty healthy male SD rats aged 6 weeks were randomly divided into control group,model group,NLRP3 agonist group,and NLRP3 in-hibitor group,with 10 rats in each group.The control group received no intervention,while acute hemorrhoid models were established in the other three groups.After successful modeling,the NLRP3 agonist group and the NLRP3 inhibitor group were administered local subcutaneous injections of NLRP3 agonist and NLRP3 inhibitor at the wound site,respectively.The model group and the control group received subcutaneous injections of equal volume of saline.The pathological characteristics of the wounds in each group were observed and wound scores were assessed.Enzyme-linked immu-nosorbent assay(ELISA)was used to detect serum levels of tumor necrosis factor(TNF)-α and in-terleukin(IL)-6.Western blot analysis was employed to detect protein expression levels of NLRP3,cAMP,and TRPV1 in the wound tissue.Real-time fluorescent quantitative polymerase chain reac-tion(qRT-PCR)was used to detect mRNA expression levels of NLRP3,cAMP,and TRPV1 in the wound tissue.Results Compared with the control group,the model group exhibited increased in-flammatory cell infiltration,pronounced edema,disrupted tissue structure,and connective tissue hy-perplasia in the wound tissue.When compared to the model group,the NLRP3 agonist group showed an increase in inflammatory cell infiltration,whereas the NLRP3 inhibitor group demonstrated a re-duction in inflammatory cell infiltration.After intervention,compared with the control group,the model group had elevated wound scores and serum levels of TNF-α and IL-6.Additionally,the rela-tive protein expression levels of NLRP3,cAMP,and TRPV1,as well as the mRNA expression levels of NLRP3,cAMP,and TRPV1 in the wound tissue,were increased in the model group(P＜0.05).Following intervention,when compared to the model group,the NLRP3 agonist group showed an in-crease in all aforementioned indicators,whereas the NLRP3 inhibitor group exhibited a decrease in these indicators(P＜0.05).Conclusion The NLRP3 inflammasome may participate in the develop-ment and progression of acute hemorrhoids in rats by activating the cAMP/TRPV1 signaling pathway.
