IDENTIFICATION OF THE TICK AUTOPHAGY MOLECULE INHIBITING THE PROLIFERATION OF BABESIA MICROTI
10.3969/j.issn.1005-0507.2025.02.005
- VernacularTitle:抑制田鼠巴贝斯虫增殖的蜱自噬分子的鉴定
- Author:
Feng-Jun GONG
1
;
Jie CAO
;
Yong-Zhi ZHOU
;
Ya-Nan WANG
;
Hou-Shuang ZHAHG
;
Jin-Lin ZHOU
Author Information
1. 中国农业科学院上海兽医研究所,农业农村部动物寄生虫学重点实验室,上海 200241
- Keywords:
Babesia microti;
Haemaphysalis longicornis;
Autophagy;
ATG8
- From:
Acta Parasitologica et Medica Entomologica Sinica
2025;32(2):93-98
- CountryChina
- Language:Chinese
-
Abstract:
Objective Ticks serve as vectors for transmitting Babesia microti.However,the specific mechanism remains unclear.This study aimed to investigate the effect of tick autophagy molecules on the proliferation of Babesia microti.Methods An experimental model of infected and uninfected mice was used to collect tick materials for proteomic analysis to identify differentially expressed autophagy-related molecules in Haemaphysalis longicornis.The cloning of the HlATG8 gene,protein expression,and production of polyclonal antibodies were completed.The HlATG8 gene was then knocked down using RNAi interference technology.Results The tick autophagy molecule,HlATG8,was identified and found to be significantly upregulated in ticks infected with Babesia microti.The load of Babesia microti in ticks increased significantly following the knockdown of the HlATG8 gene.Conclusions The tick autophagy molecule in Hae.longicornis,HlATG8,inhibits the proliferation of Babesia.
