EFFECT OF PD-1 DEFICIENCY ON IMMUNE RESPONSE IN MICE INFECTED WITH TRICHINELLA SPIRALIS
10.3969/j.issn.1005-0507.2025.02.001
- VernacularTitle:PD-1缺失对旋毛虫感染小鼠体内免疫反应的影响
- Author:
Si-Meng ZHAO
1
;
Xin-Yang HUANG
;
Yi-Qi LIU
;
Yao ZHANG
;
Yan YU
;
Jing-Jing HUANG
;
Xin-Ping ZHU
;
Yu-Li CHENG
Author Information
1. 首都医科大学基础医学院人体寄生虫学教研室,北京 100069
- Keywords:
Trichinella spiralis;
Programmed death-1;
T helper 2 cell;
Eosinophils
- From:
Acta Parasitologica et Medica Entomologica Sinica
2025;32(2):65-72
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of programmed death-1(PD-1)on cell infiltration in muscle tissue and immune response types in mice infected with Trichinella spiralis.Methods C57BL/6J wild-type(WT)and PD-1 deficient(PD-1-/-)mice were infected with T.spiralis(400 muscle larvae per mouse),and samples were collected on day 35 after infection.The proportions of infiltrating inflammatory cells and fibroblasts around encapsulated larvae were assessed by immunohistochemistry.The expression levels of interferon-γ(IFN-γ),interleukin(IL)-4,IL-5,IL-13,and eotaxin in muscle tissue were measured using enzyme-linked immunosorbent assay.Peripheral blood and spleen were collected at different time points after infection.The percentages of CD4+IFN-γ+Th1 and CD4+IL-4+Th2 within CD4+T cells population in peripheral blood and spleen of mice were analyzed using flow cytometry.Results The proportions of eosinophils and fibroblasts among total infiltrating cells around the encapsulated larvae in the muscle of PD-1-/-mice were significantly lower than those in WT mice after T.spiralis infection(P＜0.01).The infected PD-1-/-mice exhibited higher proportions of macrophages,T cells and B cells in total infiltrating cells than the infected WT mice(P＜0.01).The levels of IL-4,IL-5,IL-13,and eotaxin in the muscle tissue of infected PD-1-/-mice were significantly lower than those in infected WT mice(P＜0.05).However,IFN-γ levels were not significantly different between the infected WT and PD-1-/-mice.The proportions of Th2 cells in CD4+T cells from peripheral blood and spleen of infected PD-1-/-mice were significantly lower than those in infected WT mice,whereas the proportion of Th1 cells showed no difference among the infected groups.Conclusions PD-1 deletion results in decreased expression of key chemokines of eosinophils and key cytokines of fibroblast formation,and a corresponding decrease in inflammatory cells in muscle in T.spiralis-infected mice.This effect may be associated with a diminished Th2 immune response caused by PD-1 deletion.
