POLYMORPHISM OF THE INTERNAL TRANSCRIBED SPACER SEQUENCES OF EUSTRONGYLIDES SPP.IN MONOPTERUS ALBUS
10.3969/j.issn.1005-0507.2025.01.011
- VernacularTitle:黄鳝胃瘤线虫ITS序列的多态性研究
- Author:
Ri-Hua YANG
1
;
Huan-Fu TIAN
;
Lin-Sen ZOU
;
Song-Bai WANG
;
De-Liang LI
;
Wei LIU
Author Information
1. 永州市农业科学研究所,湖南 永州 425100
- Keywords:
Monopterus albus;
Eustrongylides spp.;
ITS;
Phylogenetic relationship
- From:
Acta Parasitologica et Medica Entomologica Sinica
2025;32(1):60-64
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aim of the study is to reveal the polymorphisms of Eustrongylides spp.in Monopterus albus.Methods A total of five Eustrongylides spp.collected from five M.albus in the Yongzhou area were sampled to amplify ribosomal ITS sequence by PCR in vitro,then sequenced after extracting the DNA templates of Eustrongylides.The phylogenetic tree based on the ITS sequence was also constructed to explore the evolutionary history of Eustrongylides.Results The length of the ITS sequence of the five Eustrongylides spp.is approximately 933 bp,and the genetic differences between the isolates are 0.00％-0.90％and the genetic inheritance differences with other nematodes are 15.5％-18.20％,maintaining a certain genetic distance from other families.Conclusions The ITS sequence of Eustrongylides spp.in M.albus will be used as a genetic marker for the molecular identification of Eustrongylides spp.and will provide a basis for the prevention and control of Eustrongylides spp.in the future.
