META-TRANSCRIPTOMIC DISCOVERY FOR EMERGING TICK-BORNE VIRUSES IN WUWEI,GANSU PROVINCE
10.3969/j.issn.1005-0507.2024.04.005
- VernacularTitle:甘肃武威地区蜱类携带潜在新病毒的宏转录组研究
- Author:
Ji-Hu YANG
1
;
Lin HUANG
;
Shun-Shuai LIU
;
Ai-Xia HUANG
;
Yi-Wen LIU
;
Jia-Fu JIANG
Author Information
1. 安徽医科大学公共卫生学院流行病与卫生统计学系,合肥 230032;军事科学院军事医学研究院,病原微生物生物安全全国重点实验室,北京 100071
- Keywords:
Meta-transcriptome;
Tick-borne viruses,Gansu;
Ticks
- From:
Acta Parasitologica et Medica Entomologica Sinica
2024;31(4):216-223
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aim of the paper is to monitor the natural infection of the viruses in ticks from the Gansu Province and elicit the risk of tick-borne viral disease transmission in the Silk Road Economic Zone.Methods The Wuwei area,where the Loess Plateau,the Tibetan Plateau and the Meng-Xin Plateau converge,was selected as a surveillance site to collect off-host or parasitic tick samples and detect the potential viruses harbored in them with meta-transcriptomic sequencing.The clean reads were assembled and annotated to explore the diversity of viruses under the genome phylogenetic analyses,identifying potential novel viruses.Results A total of 98 ticks of 5 species in 3 genera were sampled,including Dermacentor silvarum,Dermacentor nuttalli,Hyalomma asiaticum,Hyalomma scupense and Haemaphysalis kolonini.Among these tick samples,Mivirus shows the highest abundance,followed by Orthopoxvirus and Orthonairovirus.The assembled viral sequences were identical to Bole tick virus 1,Bole tick virus 3,Changping tick virus 1 and Taishun tick virus.Meanwhile,a novel subspecies of Bole tick virus 4 with 96.92%identity to the closest Flaviviridae sp.was detected and provisionally named"Bole-like tick virus 4"with pending characterization by isolation or other approaches.Conclusions The occurrence of diverse tick-borne viruses and potential novel viruses in the surveillance sites indicates that surveillance and preventive measures are warranted.
