PRELIMINARY STUDY ON THE TOXIC ACTIVITY OF NANOEMULSION-PERILLA ESSENTIAL OIL AGAINST NON-ADULT STAGES OF CULEX PIPIENS PALLENS
10.3969/j.issn.1005-0507.2024.02.007
- VernacularTitle:紫苏精油纳米乳液对非成虫期淡色库蚊杀灭活性的初步探讨
- Author:
Wen-Xing ZHAGN
1
;
Ying ZHANG
;
Rui-Min ZHANG
;
Rui LI
;
Bo SHEN
Author Information
1. 南京医科大学基础医学院病原生物学系,江苏省现代病原生物学重点实验室,南京 211166
- Keywords:
Culex pipiens pallens;
Perilla essential oils;
Nanoemulsions;
Larvicides;
Mosquito vector control
- From:
Acta Parasitologica et Medica Entomologica Sinica
2024;31(2):104-110
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the toxic activity of perilla essential oil(PE-EO)and PE-EO nanoemulsion on larvae and pupae of Culex pipiens pallens.The findings of this study will provide a scientific basis for the development of safe and effective plant-derived mosquitoicides.Methods The larvicidal and pupicidal activity,the duration of action of PE-EO nanoemulsion against Cx.pipiens pallens,and toxicity to non-target organisms were determined using a series of laboratory bioassays.Results The LC50 of PE-EO against L1,L2,L3 larvae was 25.4,43.0,46.6 mg/L,while those of nanoemulsion-PE-EO against L1,L2,L3 larvae was＜25.0,35.1,38.1 mg/L respectively.At all larvae stages,the larvicidal activity of the nanoemulsion was significantly greater than that of pure EO at the same concentration.In pupal stage,the mortality of PE-EO at 24 h and 48 h were 1.3％and 2％,while that of nanoemulsion-PE-EO were 34.7％and 40％.The larvicidal activity of the essential oil(EO)is limited to a period of 1-2 days,whereas the nanoemulsion can maintain its efficacy for up to 5 days.Neither the EO nor the nanoemulsion exhibited any toxicity towards aquatic plants.However,the toxicity of the PE-EO mixture towards fish was significantly reduced following nanoemulsion encapsulation.Conclusion In comparison to PE-EO,the nanoemulsion displays enhanced stability,which has resulted in augmented killing activity against mosquito larvae and a reduction in toxicity to non-target organisms.Nanoemulsion-PE-EO has the potential to serve as an environmentally friendly,sustainable and efficacious larvicide for mosquito vector control.
