EFFECTS OF ENHANCED DULBECCO'S MODIFIED EAGLE MEDIUM ON BLASTOCYSTIS HOMINIS CULTURE
10.3969/j.issn.1005-0507.2024.02.002
- VernacularTitle:改良DMEM培养基对人芽囊原虫的培养效果观察
- Author:
Ming-Chao ZHU
1
;
Ya ZHU
;
Hui-Zhen YUAN
;
Fei-Bo GUO
;
Jun WANG
;
Ling SHU
Author Information
1. 天门市第一人民医院检验科,湖北天门 431700
- Keywords:
Blastocystis hominis;
Enhanced DMEM;
Iodine staining;
Morphological observation
- From:
Acta Parasitologica et Medica Entomologica Sinica
2024;31(2):72-77
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of enhanced Dulbecco's modified Eagle's medium(DMEM)on the cultivation of Blastocystis hominis.Methods A total of 329 clinical samples were selected to compare the growth of B.hominis using enhanced DMEM and the iodine solution direct smear method.The morphology,minimum detection limit and growth curve of B.hominis were compared between the conventional DMEM culture medium and the enhanced DMEM culture method.Results In 329 clinical specimens,the positive rate for B.hominis was 5.17％(17/329)in the enhanced DMEM culture and 1.52％(5/329)in the iodine solution stained smears;the difference was statistically significant(P＜0.01).Conclusion The enhanced DMEM culture method can be used for the proliferation and culture of B.hominis in vitro.
