ESTABLISHMENT AND APPLICATION OF A QPCR-BASED METHOD FOR WOLBACHIA GENOTYPE AND DENSITY DETECTION IN AEDES ALBOPICTUS
10.3969/j.issn.1005-0507.2024.01.004
- VernacularTitle:qPCR检测白纹伊蚊携带Wolbachia分型及密度方法的建立与应用
- Author:
Yi-Ping XIONG
1
;
Ning-Xin ZHOU
;
Guo-Rui LIANG
;
Jian-Hang LI
;
Bo LI
;
Wan-Rong HAN
;
Dan XING
;
Xiao-Xia GUO
;
Yu-Ting JIANG
;
Tong-Yan ZHAO
Author Information
1. 军事科学院军事医学研究院, 病原微生物生物安全全国重点实验室, 媒介生物危害和自然疫源性疾病北京市重点实验室, 北京 100071
- Keywords:
Aedes albopictus;
Wolbachia;
qPCR;
Tetracycline
- From:
Acta Parasitologica et Medica Entomologica Sinica
2024;31(1):19-27
- CountryChina
- Language:Chinese
-
Abstract:
Objective Naturally,Aedes albopictus carries both the A and B supergroups of Wolbachia strains,which are symbiotic bacteria that can influence the transmission of mosquito-borne diseases by inhibiting the ability of their host to transmit viruses or by reducing the population density of their host,and the strength of this effect is closely related to its density in host cells.In this study,an accurate qPCR-based method for detecting Wolbachia genotype and density in mosquitoes was established.Methods The wsp gene of Wolbachia carried by Ae.albopictus was cloned and sequenced,and specific primers for the non-conserved regions of the w AlbA and w AlbB wsp genes were designed.Results The application of this method showed great sensitivity and specificity.The primers did not cross-react between the two Wolbachia supergroups.Further,with this method,it was possible to detect decreases in Wolbachia density in host cells following treatment with tetracycline at different concentrations,and the optimal tetracycline treatment concentration was determined to be 0.1 mg/mL.Conclusion This method can provide technical supports for studies of the effects of Wolbachia on physiological reproduction and innate immunity in mosquitoes.
