INVESTIGATION OF ARBOVIRUSES CARRIED BY VECTOR AEDES MOSQUITOES IN THE URBAN AREAS OF XISHUANGBANNA PREFECTURE,YUNNAN PROVINCE DURING THE DENGUE FEVER EPIDEMIC SEASON OF 2018
10.3969/j.issn.1005-0507.2023.04.003
- VernacularTitle:2018年登革热流行季节云南西双版纳州城区媒介伊蚊携带虫媒病毒调查
- Author:
Xiao-Fang GUO
1
;
Ke-Mei ZHOU
;
Zi-Long CHEN
;
Xiang XU
;
Xue-Mei LAN
;
Xue-Fei WANG
;
Li-Min DONG
;
Yang GAO
;
Hong-Bin LI
;
Wei-Ping LI
Author Information
1. 云南省寄生虫病防治所, 云南省虫媒传染病防控研究重点实验室, 云南普洱 665000
- Keywords:
Arbovirus;
Insect-specific virus;
Dengue virus;
Aedes aegypti;
Aedes albopictus
- From:
Acta Parasitologica et Medica Entomologica Sinica
2023;30(4):202-209
- CountryChina
- Language:Chinese
-
Abstract:
To understand the arbovirus carried by the vector Aedes mosquito in the urban areas of Xishuangbanna Dai Autonomous Prefecture(Xishuangbanna Prefecture)during the dengue fever epidemic season in Yunnan province,real-time RT-PCR analysis for DENV serotyping and E gene detection were conducted on the RNA extracted from Aedes mosquitoes(including Ae.albopictus and Ae.aegypti)collected from August to October and DENV NS1 positive serum collected from July to November 2018 in Xishuangbanna Prefecture.A phylogenetic analysis was performed on the sequences obtained from the positive amplification products targeting Flaviviridae sp..C6/36 Ae.albopictus cells were used to isolate viruses from mosquito homogenates.Isolates with cytopathic effects(CPE)were identified by arbovirus and insect-specific virus RT-PCR and sequencing.The sequences were compared to the online GenBank database by BLAST.The DENV serotyping detection results indicated that two groups of Ae.aegypti among 51 groups of Aedes mosquitoes(1 431 mosquitoes in total)and 52 out of 54 serum samples were positive for DENV-1.One mosquito DENV-1 E gene sequence and 27 serum DENV-1 E gene sequences were obtained.The nucleotide sequence identities of E genes derived between mosquito and serum were 97.4%～100.0% and they belonged to genotype I,which had the closest genetic relationship with DENV-1 from Southeast Asian nations.Additionally,Seventeen La Tina virus(LTNV,belonging to Flaviviridae)strains and four Sarawak virus(SWKV,belonging to Alphateraviridae)strains were identified from 18 isolates using regular CPE isolated from suspensions derived from 51 groups containing vector Aedes mosquitoes,of which three strains were simultaneously positive for both LTNV and SWKV detection.The results from this study indicate that vector Aedes mosquitoes in Xishuangbanna Prefecture carry DENV,LTNV,and SWKV and that further researches on arboviruses and insect-specific viruses is urgent necessary.
